Spray dried enzyme product

a technology of enzyme products and sprays, which is applied in the field of spraying dried enzyme products, can solve problems such as the development of complex particulate enzyme products, and achieve the effects of simple and cost-effective processes and good properties

Inactive Publication Date: 2005-09-22
NOVOZYMES AS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0011] The present invention provides simple and cost effective processes for producing dry enzyme particles having good properties. DEFINITONS

Problems solved by technology

Improved properties of biological compounds such as enzymes, desired or required by industry and applications has led to development of complex particulate enzyme products, a task in which many challenging problems arise.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

Spray-drying of a high product titre protease fermentation broth.

[0153] A production size batch of protease containing fermentation broth with an dry matter content of 13% w / w was sieved through a rotary brush strainer to remove large solid particulates. The fermented micro-organism was killed by adding 0.5% Rodalon (50% Benzalkoniumchlorid) to the broth while maintaining agitation. Formic acid was then added to reduce pH to 5.5 and the broth left for an hour or more to allow the Rodalon to be effective. The broth was heated to an inlet temperature of 140° C. and introduced to a drying chamber (Niro Atomizer, SD-200-R spray drying tower) via a rotating spray nozzle. The exit temperature being 55° C. The resulting spray dried powder had an activity of 41 KNPU / g and a water content of 4.86%

example 2

Production-scale spray-drying of a high product titre cellulase fermentation broth.

[0154] A production size batch of cellulase containing fermentation broth is sieved through a rotary brush strainer to remove large solid particulates. To kill the production strain, 0.5% Rodalon (50% Benzalkoniumchlorid) is added while the broth is well agitated and the broth is left for at least one hour to allow the Rodalon to be effective. The broth introduced to a spray tower (Niro Atomizer, SD-200-R) via a rotating spray nozzle at an inlet temperature of 140° C. The exit temperature should be around 55° C. The resulting spray dried powder have an estimated activity of 20000 ECU / g and a water content estimated below 5.0% w / w.

example 3

Production-scale spray-drying of a high product titre amylase fermentation broth.

[0155] A production size batch of amylase containing fermentation broth is sieved through a rotary brush strainer to remove large solid particulates. To kill the production strain, 0.5% Rodalon (50% Benzalkonium-chlorid) is added while the broth is well agitated and the broth is left for at least one hour to allow the Rodalon to be effective. The broth is introduced to the spray tower (Niro Atomizer, SD-200-R) via a rotating spray nozzle at an inlet temperature of 140° C. The exit temperature should be around 55° C. The resulting spray dried powder have an estimated activity of 960 KNU / g and a water content estimated below 5.0% w / w.

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Abstract

The invention relates to a particle comprising an enzyme and a biomass, to a process for preparing a particle comprising spray drying an enzyme and biomass containing fermentation broth starting material, to obtain a solid particle comprising an enzyme and a biomass and to a process for preparing an enzyme containing particle comprising spray drying an aqueous enzyme containing liquid starting material to obtain a spray dried first enzyme containing particle and subsequently subjecting the first dry particle to a process selected from granulation and coating and combinations thereof to obtain a second dry enzyme containing particle.

Description

CROSS-REFERENCE TO RELATED APPLICATIONS [0001] This application is a division of U.S. application Ser. No. 09 / 675,950, filed Sep. 29, 2000, which claims priority or the benefit under 35 U.S.C. 119 of Danish application nos. PA 2000 00251, filed Feb. 17, 2000, and PA 2000 01415, filed Oct. 1, 1999 and U.S. provisional application Nos. 60 / 158,270, filed Oct. 7, 1999, and 60 / 185,206, filed Feb. 25, 2000, the contents of which are fully incorporated herein by reference.TECHNICAL FIELD [0002] This invention relates to enzyme containing particles, to processes for preparing enzyme containing particles, and to the use of enzyme containing particles in compositions and applications of such particles and compositions. BACKGROUND OF THE INVENTION [0003] Enzyme products are known and used in a variety of industries and applications. [0004] Known art for providing dry particulate enzyme products includes: [0005] Spray dried products, as described in Michael S. Showell (editor); Powdered deterge...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): A23K1/165C11D3/386C11D11/02C12N9/26C12N9/42
CPCA23K1/1653C11D3/38609C11D3/38672C12Y302/01004C12N9/2408C12N9/2414C12N9/2437C11D11/02A23K20/189
Inventor JORGENSEN, JORGEN TOPPJACOBSEN, CARSTENHANSEN, KIM UHREJORGENSEN, ANDERSOFTELUND, DANBACH, POULSONDERGAARD, GUSTAV BORUP
Owner NOVOZYMES AS
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