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Method and means for detecting inflammatory processes

a technology of inflammatory processes and methods, applied in the field of methods and means for detecting inflammatory processes, can solve the problems of difficult or at least time-consuming sampling in the sitting, for instance in the gastro-intestinal tract and in the urinary tra

Inactive Publication Date: 2005-12-22
PETTERSSON ANDERS +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This method allows for precise quantification of nitrogen oxide production at the site of administration, distinguishing it from background levels, thereby enhancing the utility of nitrogen oxide as a reliable inflammation marker for conditions like ulcerative colitis, asthma, and other inflammatory processes.

Problems solved by technology

In situ sampling, for instance in the gastro-intestinal tract and in the urinary tract, often is difficult or at least time-consuming.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0023] Terminally N-labeled arginine. L-[guanido-15N2]-arginine is commercially available from ICN Pharmaceuticals, Inc. (Costa Mesa, Calif.) and Tracer Technology, Inc. (Somerville, Mass.). Fully 15N-labeled arginine can also be used. It can be produced by Corynebacterium herculis or Brevibacterium flavum strains carrying the recombinant DNA pCarg11 or pCarg110 according to the method disclosed in U.S. Pat. No. 5,017,482, which is hereby incorporated by reference, with the proviso that 15N3 ammonium sulphate is substituted for ammonium sulphate as the nitrogen source. L-[guanido-15N2]-arginine can be used in form of the free base or a pharmaceutically acceptable salt, such as the hydrochloride or aspartate.

example 2

[0024] Enteric tablet. An enteric tablet for release in the small intestine and the upper large intestine can be prepared according to EP 0 502 092 A1 by substituting a terminally N-labeled arginine for any of the glucocorticoids disclosed therein. A suitable amount of terminally N-labeled arginine, such as L-[guanido-15N2]-arginine, is 50 mg, in the form of the free base or a pharmaceutically acceptable salt thereof, such as the hydrochloride.

example 3

[0025] Suppository. A suitable type of suppository can be made by use of the Salazopyrin® EN, Pharmacia & Upjohn, enema composition in which the active principle sulfasalazine (500 mg) is exchanged for 50 mg L-[guanido-15N2]-arginine and 450 mg of suitable neutral constitutents, such as calcium carbonate.

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PUM

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Abstract

A method of detecting inflammatory processes comprises (a) administering per-orally or per-rectally a composition comprising a diagnostically effective amount of L-[guanido-15N2]-arginine, L-[guanido-15N]-arginine, their mixtures and pharmaceutically acceptable salts thereof, and a pharmaceutically acceptable carrier; (b) collecting a sample of exhaled air, saliva or urine; (c) determining 15NO in the air sample or its transformation products 15N-nitrite and / or 15N-nitrate in the saliva sample or urine. Also disclosed is a corresponding diagnostic composition for use in diagnosing inflammatory processes and a method for its manufacture.

Description

FIELD OF THE INVENTION [0001] The present invention relates to a method for determining nitrogen oxide formed in inflammatory processes, to a means for carrying out the method and to a method of manufacture of the means. BACKGROUND OF THE INVENTION [0002] Nitrogen oxide (NO) has important biological functions. It is the structurally simplest mediator in the human body as well as one of the most important weapons of its immune defense. In its later function it is excreted by activated macrophages to kill foreign microorganisms and cells recognized as foreign. In this second capability nitrogen oxide may be considered an inflammation marker and has been recognized as such in, for instance, inflammatory processes in the gastro-intestinal tract, such as ulcerative colitis and Crohn's disease and celiac disease. In the lung it may assume both roles. It is recognized that levels of nitrogen oxide excretion are raised in asthmatics. Thus it may be considered a marker for asthma which also ...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): A61B5/08A61K49/00A61K51/02A61K51/12G01N33/487G01N33/493G01N33/497G01N33/50
CPCA61B5/0813A61K51/1206A61K51/02A61B5/412
Inventor PETTERSSON, ANDERSLUNDQVIST, THOMAS
Owner PETTERSSON ANDERS