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Composition for controlling increase in blood glucose

a technology of blood glucose and composition, applied in the field of composition for controlling the increase of blood glucose, can solve the problems of long postprandial hyperglycemia, use can induce some gastrointestinal side effects, and use can prolong postprandial hyperglycemia, so as to reduce glucose and cholesterol absorption, hinder 2-deoxyglucose uptake, and increase blood glucose and insulin levels

Inactive Publication Date: 2011-08-04
NUC ELECTRONICS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0007]Prolonged postprandial hyperglycemia is a detrimental factor for type 2 diabetes and obesity. The benefit of green tea extract (GTE) consumption still requires confirmation. We report the effects of circulating green tea catechins on blood glucose and insulin levels. Oral glucose loading 1 h after GTE ingestion in humans led to higher blood glucose and insulin levels than in control subjects. Gallated catechins were required for these effects, although within the intestinal lumen they have been known to decrease glucose and cholesterol absorption. Treatment with epigallocatechin-3-gallate hindered 2-deoxyglucose uptake into liver, fat, pancreatic beta-cell, and skeletal muscle cell lines. Glucose intolerance was ameliorated by gallated catechin-deficient GTE or GTE mixed with polyethylene glycol, which was used as an inhibitor of intestinal absorption of gallated catechins. These findings suggest that gallated catechin when it is in circulation elevates blood glucose level by blocking normal glucose uptake into the tissues, resulting in secondary hyperinsulinemia, whereas it decreases glucose entry into the circulation when they are inside the intestinal lumen. These findings encourage the development of non-absorbable derivatives of gallated catechins for preventative treatment of type 2 diabetes and obesity, which would specifically induce only positive luminal effect.

Problems solved by technology

In MODY-2 diabetes, functional defects in glucokinase genes restrict hepatic glucose uptake, bringing about prolongation of postprandial hyperglycemia (Jiang et al, 2008), and eventually result in beta-cell overload.
Although amylase inhibitors or glucosidase inhibitors are available to date to decrease postprandial hyperglycemia, the usage can induce some gastrointestinal side effects, as they block the conversion to monosaccharide.
However, the debate over whether GTE or EGCG is actually applicable for the prevention or treatment of human obesity and type 2 diabetes has not been settled (Anderson & Polansky, 2002; Fukino et al, 2005; Naftalin et al, 2003).
Daily consumption of green tea by diabetic humans over a period of several months was recently revealed to be ineffective in alleviating blood glucose levels, HbA1C levels, insulin resistance and inflammation markers (Fukino et al, 2005).
Nevertheless, a certain proportion of the ingested catechins is inevitably absorbed into the blood and acts at other sites within the body.
In addition, as rats have very low oral bioavailability of GTE, during the experiment, it is more difficult to detect the effect of GTE after GTE is absorbed in the circulation.

Method used

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  • Composition for controlling increase in blood glucose
  • Composition for controlling increase in blood glucose
  • Composition for controlling increase in blood glucose

Examples

Experimental program
Comparison scheme
Effect test

example 1

Materials and Methods

Example 1.1

Materials

[0053]Dulbecco's modified Eagle's medium (DMEM), phosphate-buffered saline (PBS), fetal bovine serum (FBS) and fetal calf serum (FCS) were purchased from Gibco (Carlsbad, Calif.). RPMI-1640 medium was purchased from Welgene (Daegu, Korea). Polyethylene glycol (PEG; Novasyn TG hydroxy resin) was delivered from Novabiochem (Darmstadt, Germany) for animal study. PEG for human study was kindly gifted from Taejoon (Seoul, Korea). Green tea leaves (BOSUNG SEIJAK) were purchased from Bosung green tea Co. (Jeonnam, Korea). Epigallocatechin-3-gallate (EGCG), epicatechin-3-gallate (ECG), epigallocatechin (EGC) and epicatechin (EC) were purchased from Sigma-Aldrich (St. Louis, Mo.). All other chemicals were obtained from Sigma-Aldrich.

example 1.2

Cell Culture

[0054]Rat L6 myoblasts were cultured in low-glucose DMEM with 10% (v / v) FBS until they reached 80% confluency. To induce differentiation, cells were further cultured in DMEM (24.9 mM glucose) containing 2% FBS for 7 days. Cell viability was assessed by the trypan blue viability test. Myogenic differentiation to myotube status was evaluated both morphologically and biochemically. Mouse 3T3-L1 preadipocytes were grown to confluence at 37° C. in 35-mm culture dishes in DMEM containing 10% FCS with no added biotin or pantothenate in incubators equilibrated with 5% CO2. At two days post-confluence (day 0), differentiation was induced with methylisobutylxanthine (0.5 mM), dexamethasone (0.5 μM) and insulin (5 μg / ml) in DMEM containing 10% FBS. On day 2, methylisobutylxanthine and dexamethasone were removed and insulin treatment was continued for 2 additional days. On day 4 and thereafter, DMEM (without insulin supplementation) plus 10% FBS was replaced every 2 days. Cells (2×1...

example 1.3

Preparation of GTE

[0055]For animal studies, 20 g of green tea leaves were added to 1,000 ml of nanopure water. After being stirred for 5 min at 80° C., the tea leaves were removed by filtration using filter paper (Advantec 2 filter paper, Hyundai micro Co., Seoul, Korea) under reduced pressure. The extract was dried by lyophilization. (A total of 3 g of GTE was harvested, in which EGCG, ECG, EGC and EC were ˜100, 53, 56 and 31 mg / g GTE, respectively). An equal amount of the GTE solution was mixed with 2 g PEG bead for 5 min at room temperature. After filtration, the supernatant was lyophilized (EGCG, ECG, EGC and EC were ˜26, 14, 42 and 25 mg / g GTE, respectively) to obtain GC-deficient GTE (GTE-GC). The results indicated that the resin pretreatment preferentially reduced GC from the GTE solution. For the human study, 30 g of green tea leaves were added to 500 ml of nanopure water. After being stirred for 3 min at 80° C., tea leaves were removed by filtration, remaining a 350 ml solu...

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Abstract

The present application describes a composition for blood glucose control containing green tea extracts (GTE) with gallated catechins (GC) and macromolecule to prevent the intestinal absorption of GC.

Description

CROSS-REFERENCE TO RELATED APPLICATIONS[0001]The present application claims the benefit of priority to co-pending U.S. Provisional Application Ser. No. 61 / 083,135, filed Jul. 23, 2008, which is incorporated herein by reference in their entirety.BACKGROUND OF THE INVENTION[0002]1. Field of the Invention[0003]The present invention relates to catechin-related compositions to lower postprandial blood glucose elevation. The present invention also relates to functional foods for blood glucose control containing green tea extracts (GTE) with gallated catechins (GC) and macromolecules that prevent intestinal absorption of GC. One purpose of this invention is to take blood glucose lowering compositions after a meal without any side effects by inhibiting intestinal glucose absorption as well as lipid absorption using a different mechanism from available hypoglycemic agents.[0004]2. General Background and State of the Art[0005]Type 2 diabetes is characterized by two main features: peripheral i...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): A61K36/82A61P3/08A23L33/20
CPCA23L1/3084A23V2002/00A61K36/82A23L1/307A23L1/3002A23V2200/328A23V2250/214A23V2250/5116A23L33/105A23L33/20A23L33/25A61K31/25A61P3/08A61P3/10
Inventor SONG, DAE-KYULEE, JINHO
Owner NUC ELECTRONICS
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