Gene associated with non-biological stress resistance, and transformed plant

a technology of non-biological stress resistance and genes, applied in the field of genes associated with non-biological stress resistance and transformed plants, can solve the problems of dramatic reduction of crop production, abiotic stress is a limiting factor, and plants are constantly faced with various adverse environmental factors, so as to improve the tolerance to abiotic stress

Inactive Publication Date: 2016-09-15
IND ACADEMIC COOP FOUND YONSEI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patent is about a product that helps plants tolerate stress caused by things like drought or high temperatures. The product can be applied to plant cells or whole plants to make them more resilient.

Problems solved by technology

Due to their sessile nature, higher plants are constantly faced with various adverse environmental factors, including drought, high salt, heavy metals, cold, heat shock, and ozone, during their whole life span.
These abiotic stresses are a limiting factor for the growth and development of crop plants.
Water deficiency causes dramatic reduction of crop production globally, and the decreasing availability of fresh water may pose a future threat to humans and higher plants.
However, for stress tolerance or sensitivity, our knowledge concerning the biological functions of stress-related genes in higher plants is still rudimentary.
Until now, it has been known that proteasome-dependent degradation of the substrate occurs only when polyubiquitin consisting of at least four ubiquitins is attached to the substrate; however, it would be controversial since these results were obtained from in vitro experiments.

Method used

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  • Gene associated with non-biological stress resistance, and transformed plant
  • Gene associated with non-biological stress resistance, and transformed plant
  • Gene associated with non-biological stress resistance, and transformed plant

Examples

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Experimental Methods

Isolation of the Genes

[0063]The present inventors have isolated the AtAIRP2 genes inducible by ABA hormone and abiotic stresses from cDNAs of Arabidopsis thaliana. 10-day-old Arabidopsis thaliana seedlings were grinded with liquid nitrogen in mortar. The powders were added with 2 ml of an extraction buffer (4 M guanidine-HCl 20 mM, 10 mM EDTA, 10 mM EGTA (USB), 0.5% Sarkosyl (SIGMA), pH 9) and β-mercaptoethanol (SIGMA-ALDRICH) per 1 g of the powder for extraction. The extraction resultant was transferred to new conical tube, suspended with an equal volume of PCI (phenol:chloroform:isoamyl alcohol=25:24:1), vortexed for 5 min and centrifuged at 3,500 rpm for 25 min (Hanil centrifuge, HA-1000-3). After centrifugation, the upper organic solvent phase was removed. The extract was resuspended with an equal volume of PCI, vortexed and centrifuged twice. Then, the lower aqueous phase was undergone twice ethanol precipitation and once LiCl precipitation to isolate RNA. R...

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Abstract

The present invention relates to a composition for enhancing non-biological stress resistance in plants and a composition for accelerating germination. A nucleotide sequence of the present invention is involved in the resistance against the drying stresses in plants, and a transformed plant in which the nucleotide sequence is overexpressed has prominent resistance against various kinds of non-biological stress, including drought stress. In addition, the nucleotide sequence of the present invention is involved in ABA hormone sensitivity in plants, and germination is greatly improved in a plant in which the nucleotide sequence expression is suppressed. Therefore, the composition of the present invention can be useful as new functional crops regardless of the climate of the cultivation area, or as seeds for long-term storage with an increased storage period.

Description

BACKGROUND OF THE INVENTION[0001]1. Field of the Invention[0002]The present invention relates to a gene implicated in abiotic stress tolerance and growth promotion and a method for improving abiotic stress tolerance and promoting growing of transformed plants with the same.[0003]2. Description of the Related Art[0004]Due to their sessile nature, higher plants are constantly faced with various adverse environmental factors, including drought, high salt, heavy metals, cold, heat shock, and ozone, during their whole life span. These abiotic stresses are a limiting factor for the growth and development of crop plants. Water deficiency causes dramatic reduction of crop production globally, and the decreasing availability of fresh water may pose a future threat to humans and higher plants. Plants have diverse defense strategies to enhance their tolerance to transient and long-term water shortages by triggering signaling network pathways and inducing stress-responsive genes. The cellular a...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): C12N15/82
CPCC12N15/8271C12N15/8293C12N15/8273C07K14/415C12N9/93C12Y603/02019
Inventor KIM, WOO TAEKRYU, MOON YOUNGCHO, SEOK KEUN
Owner IND ACADEMIC COOP FOUND YONSEI UNIV
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