Gene associated with non-biological stress resistance, and transformed plant
a technology of non-biological stress resistance and genes, applied in the field of genes associated with non-biological stress resistance and transformed plants, can solve the problems of dramatic reduction of crop production, abiotic stress is a limiting factor, and plants are constantly faced with various adverse environmental factors, so as to improve the tolerance to abiotic stress
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Isolation of the Genes
[0063]The present inventors have isolated the AtAIRP2 genes inducible by ABA hormone and abiotic stresses from cDNAs of Arabidopsis thaliana. 10-day-old Arabidopsis thaliana seedlings were grinded with liquid nitrogen in mortar. The powders were added with 2 ml of an extraction buffer (4 M guanidine-HCl 20 mM, 10 mM EDTA, 10 mM EGTA (USB), 0.5% Sarkosyl (SIGMA), pH 9) and β-mercaptoethanol (SIGMA-ALDRICH) per 1 g of the powder for extraction. The extraction resultant was transferred to new conical tube, suspended with an equal volume of PCI (phenol:chloroform:isoamyl alcohol=25:24:1), vortexed for 5 min and centrifuged at 3,500 rpm for 25 min (Hanil centrifuge, HA-1000-3). After centrifugation, the upper organic solvent phase was removed. The extract was resuspended with an equal volume of PCI, vortexed and centrifuged twice. Then, the lower aqueous phase was undergone twice ethanol precipitation and once LiCl precipitation to isolate RNA. R...
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