Novel lactobacillus plantarum and composition comprising same

a technology of lactobacillus and plantarum, applied in the field of new lactobacillus plantarum, can solve the problems of allergic reactions, increase of mortality rate, decrease of gain rate,

Active Publication Date: 2017-01-26
CJ CHEILJEDANG CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

Lactobacillus plantarum CJLP133 effectively adheres to intestinal epithelial cells, enhances immune responses, and balances the Th1 / Th2 immune response, offering improved treatment options for enteric diseases, atopic dermatitis, and autoimmune conditions, with demonstrated superiority over other lactic acid bacteria in cytokine production and immune regulation.

Problems solved by technology

Bacteria-caused diarrhea in livestocks leads to the decrease of rate of gain and increase of mortality rate.
However, the use of antibiotics in feeds is being further regulated and organic livestock husbandry is recommended due to problems occurring from antibiotic resistant-bacteria and the antibiotic-remains within the animals.
In the case which Th2 cells are over activated, the production of IgE antibodies increases, and may cause allergic responses to some proteins (pollen, food) which were not as harmful before.

Method used

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  • Novel lactobacillus plantarum and composition comprising same
  • Novel lactobacillus plantarum and composition comprising same
  • Novel lactobacillus plantarum and composition comprising same

Examples

Experimental program
Comparison scheme
Effect test

example 2

Experiment Investigating Acid-Resistance and Bile Acid-Resistance of Lactobacillus plantarum CJLP133 Using Artificial Gastric Juice and Artificial Bile

[0083]The acid-resistance experiment was performed using the artificial gastric juice modified and made referring to an experiment from Kobayashi et al (Kobayashi et al., Studies on biological characteristics of Lactobacillus: II. Tolerance of the multiple antibiotic resistance strain, L. casei PSR3002, to artificial digestive fluids. Japan Journal of Microbiology, Volume 29, July 1974, pages 691-697). In detail, the artificial gastric juice was made by adjusting the pH of MRS liquid medium to pH 2.5 using 1N HCl and adding 100 unit / ml of pepsin followed by sterilisation.

[0084]The isolated Lactobacillus plantarum CJLP133 as described in Example 1 was cultured in MRS liquid medium at 37° C. for 18 hours and then centrifuged for precipitation. Then the precipitation was washed with sterilised 0.85% NaCl twice. Then 107 cfu / ml of the bac...

example 3

Experiments Testing Intestinal Epithelial-Adherence of Lactobacillus plantarum CJLP133

[0089]Animal cell line HT-29 was provided by the Korean Cell Line Bank (KCLB) in order to test intestinal epithelial-adherence, and the methods were used described in the research of Kim et al and Hirano et al (Kim et al., Probiotic properties of Lactobacillus and Bifidobacterium strains isolated from porcine gastrointestinal tract, Applied Microbiology and Biotechnology, Volume 74, April 2007, pages 1103-1111, Hirano et al., The effect of Lactobacillus rhamnosus on enterohemorrhagic Escherichia coli infection of human intestinal cells in vitro, Microbiology and Immunology, Volume 47, 2003, pages 405-109).

[0090]HT-29 was cultured in RPMI 1640 (Gibco, USA) medium added with heat deactivated 10% Fetal Bovine Serum, 1% L-Glutamine, penicillin G (100 IU / mL) and streptomycin (100 mg / mL) at 5% CO2, 37° C. In order to test adherence and detachment, 1.0×105 cell / ML of HT-29 cells were plated on wells of a ...

example 4

Safety Assessment of Lactobacillus plantarum CJLP133

[0094]Hemolysis test, gelatin liquefaction test, hazardous metabolite (ammonification) test and phenylalanine deaminase test were performed according to the safety assessment methods suggested by the standard of Korea Biotechnology Industry Organization to assess safety of the bacteria isolated from the Example 1. The obtained result is summarized in table 2.

TABLE 2Safety assessment for Lactobacillus plantarum CJLP133TestsgelatinphenylalanineBacterialiquefactiondeaminasehemolysisammonificationCJLP133negativenegativeα-hemolysis,negativesafe

[0095]Based on the above result, Lactobacillus plantarum CJLP133 was found negative in the gelatin liquefaction test, hazardoug metabolite (ammonification) test, phenylalanine deaminase test. Hemolysis test showed α-hemolysis which is irrelevant with pathogenic bacteria. Accordingly, Lactobacillus plantarum CJLP133 was confirmed as being safe for administration to a human being.

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Abstract

The present invention provides Lactobacillus plantarum CJLP133 KCTC 11403BP, a composition for treating intestinal diseases comprising the lactic acid bacteria, and a composition for enhancing immunity comprising the lactic acid bacteria.

Description

CROSS REFERENCE TO RELATED APPLICATIONS[0001]This application is a continuation of application Ser. No. 13 / 386,459 filed Jan. 23, 2012 which claims the benefit of PCT Application No. PCT / KR2009 / 004930, filed on Sep. 2, 2009, which claims the benefit of Korean Application No. KR 10-2009-0067015, filed on Jul. 22, 2009, the contents of all of which are incorporated herein by reference.TECHNICAL FIELD[0002]The present invention relates to a novel Lactobacillus plantarum, and compositions comprising the same. More particularly, the present invention relates to a novel Lactobacillus plantarum helpful for the prevention / treatment of an enteric disease and an immune disease and a composition comprising the same.BACKGROUND ART[0003]Lactic acid bacteria which can be found in traditional fermented food such as ‘Kimchi’ is in a symbiotic relationship with human within the digestive tract, and digests fibers and composite protein. These microorganisms contribute to the digestive environment wit...

Claims

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Application Information

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Patent Type & AuthorityApplications(United States)
IPC IPC(8): A61K35/747A23K1/00A23L1/30A61K8/99A61Q19/00
CPCA61K35/747A61K8/99A23Y2220/67A23L33/135A23K10/18A61Q19/00A23V2002/00A61K35/74A61P1/04A61P35/00A61P37/04A61P37/06A61P37/08A61P43/00C12R2001/25C12N1/205A23V2400/169A23V2200/3204A23V2200/324A23V2200/304A23V2200/308A23K10/16A23L33/00C12N1/20C12R2001/225
InventorKIM, BONG JOONJUNG, HEON WOONGHWANG, SE HEELEELEE, KANG-PYOHWANG, KWANG WOOWON, TAE JOON
OwnerCJ CHEILJEDANG CORP