Process of producing monoterpenes

Inactive Publication Date: 2020-04-02
C3 BIO TECH LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

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Benefits of technology

[0070]As such, there is also provided the use of a bacterial mTS to improve the purity of a monoterpene and/or derivative thereof obtained from GPP. In embodiments, the monoterpene

Problems solved by technology

However, the resulting monoterpene yields are relatively low.
However, the use of such plant mTS has associated disadvantages.
This limited solubility has proved to be a bottleneck in the production of monoterpenes as the maj

Method used

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  • Process of producing monoterpenes
  • Process of producing monoterpenes
  • Process of producing monoterpenes

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Embodiment Construction

[0087]The present invention will now be further described with reference to the following figures which show:

[0088]FIG. 1: GC-MS analysis using bacterial 1,8-cineole synthase (bCinS) and bacterial linalool synthase (bLinS). A) bCinS platform product profile. B) bCinS conversion of GPP (20 mM). C) 1,8-cineole standard (0.1 mg / ml). D) bLinS platform product profile. E) bLinS conversion of GPP (20 mM). F) R-linalool standard (0.1 mg / ml). G) cis- and trans-nerolidol standards (0.1 mg / ml). IS=internal standard (sec-butyl benzene).

[0089]FIG. 2: Structure of bCinS-FNPP complex. Superposition of plant cineole synthase (dark) and bCinS (light). The N-terminal and C-terminal domains of the plant CinS are labelled. An arrow indicates the conformational difference in the helix-break motif.

[0090]Materials and Methods

[0091]Expression and Purification of Bacterial 1, 8-Cineole Synthase (bCinS) and Bacterial Linalool Synthase (bLinS)

[0092]The full-length genes coding for 1,8-cineole synthase (WP_00...

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Abstract

The present invention relates to a process of producing a monoterpene and/or derivatives thereof. The process comprises the steps of: a) providing a host microorganism genetically engineered to express a bacterial monoterpene synthase (mTS); and b) contacting geranyl pyrophosphate (GPP) with said bacterial mTS to produce said monoterpene and/or derivatives thereof. The present invention also relates to a microorganism for use in producing a monoterpene and/or derivatives thereof and a recombinant microorganism adapted to conduct the step of converting geranyl pyrophosphate (GPP) into a monoterpene and/or derivatives thereof by expression of a bacterial mTS. It was shown to produce 1,8 cineole using 1,8 cineole synthase and to produce linalool using linalool synthase, both from Streptomyces clavuligerus.

Description

FIELD OF THE INVENTION[0001]The present invention relates to a process of producing monoterpenes. In particular, the present invention relates to a process of producing monoterpenes, for example in a host microorganism, by the action of a bacterial monoterpene synthase.BACKGROUND TO THE INVENTION[0002]Terpenoids (also called isoprenoids) are the most abundant and largest class (>75000) of natural products. Most commonly found in plants, their biological roles are multitude ranging from species to species communication to intracellular signalling and defence against predatory species. They have a wide range of applications and are used in pharmaceuticals, herbicides, flavourings, fragrances and biofuels. Due to the broad commercial interest for terpenoids, efforts to synthesize them by synthetic biology routes have gathered pace in recent years.[0003]Terpenoid substrates are synthesized from the C5 isoprene building blocks, namely dimethylallyl pyrophosphate (DMAPP) and isopenteny...

Claims

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Application Information

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IPC IPC(8): C12P17/06C12P7/04C12N9/88
CPCC12Y402/03025C12Y402/03108C12N9/88C12P7/04C12Y402/03026C12P17/06C12P5/002C12P5/007C12P5/026C12P17/02
Inventor KARUPPIAH, VIJAYKUMARLEFERINK, NICOLE G. H.SCRUTTON, NIGEL S.
Owner C3 BIO TECH LTD
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