The invention belongs to the technical field of biological medicines, and particularly relates to an oral soluble microneedle patch based on nano-
enzyme synergy as well as a preparation method and application of the oral soluble microneedle patch. A
mixed solution containing CMCS, PVA, Se-SCDs, APS and VEGF is used as a microneedle tip layer matrix, a
mixed solution containing CMCS, PVA and Se-SCDs is used as a microneedle substrate layer matrix, and the oral soluble microneedle patch with gradient degradation capacity is prepared. The tip of the microneedle patch removes
active oxygen through Se-SCDs, APS promotes immunoregulation, VEGF accelerates
angiogenesis, and healing of ulcer wounds is promoted; and the microneedle patch substrate simultaneously plays the functions of resisting
bacteria and regulating oral
microbiome. Due to the gradient degradation design, the micro-needle patch can protect the
wound surface and reduce external stimulation, and meanwhile, the gradual degradation of the micro-needle substrate reduces
foreign body sensation in the
oral cavity; the tip of the microneedle is slowly degraded, so that long-range release of Se-SCDs, APS and VEGF is realized, and synergistic treatment is realized. Experiments prove that the oral
ulcer healing rate is remarkably improved, and high
biological safety and clinical transformation value are achieved.