The invention discloses a microphone gain adjustment method, a device, a system and a storage medium. The method comprises the following steps: acquiring a volume value of a sound signal played by a loudspeaker; judging whether the volume value is greater than a preset volume threshold; and if yes, performing reduction processing on the preset microphone gain according to the volume value of the played sound signal, and setting the target gain obtained after reduction as the current microphone gain. According to the microphone gain adjustment method provided by the invention, when the volume value of the sound signal played by the loudspeaker is greater than the preset volume threshold value, the preset microphone gain is reduced, the smaller microphone gain is set as the current microphone gain, non-linear distortion generated after the sound signal with larger volume emitted by the loudspeaker is picked up and amplified by the microphone is avoided, the accurate user sound signal canbe obtained, and the effective awakening of the intelligent sound box is further ensured.