Microorganism of producing D-pantothenic acid enternal ester hydrolase and process for preparing D-pantothenic acid thereof

Abstract

The invention relates to a method used microorganism enzyme resolution DL-pantoic acid lactone to produce D-pantoic acid. It uses the D-pantoic acid lactone hydrolase strain of the fusarium, gibberella, aspergillus, penicillium, rhizopus, gliocladium, aureobasidium to ferment and culture, uses wet thallus as coarse enzyme, DL-pantoic acid lactone as substrate to produce D-pantoic acid. L- pantoic acid lactone can be reclaimed. The DL-pantoic acid lactone gained by racemization reaction can newly be used to do resolution.

Description

technical field

[0001] The invention belongs to the field of biocatalysis research, and relates to a microorganism producing D-pantoate lactone hydrolase and a method for preparing D-pantoate. Background technique

[0002] D-pantothenic acid (Pantothenic acid) is an important chiral intermediate for the manufacture of D-pantothenic acid. D-pantothenic acid is a vitamin B group substance and a component of coenzyme A. It participates in the metabolism of sugar, fat and protein. Its products are mostly D- It exists as calcium pantothenate. Calcium D-pantothenate is widely used in medicine, food and feed industry.

[0003] At present, D-pantothenic acid is generally prepared by chemical or biological resolution methods. Traditional chemical resolution methods have disadvantages such as high production cost, small production volume, and poor optical purity of products. Among the biological resolution methods, the separation of DL-pantolactone by microbial enzymatic method has...