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Biodemulsifier, its preparation method and application

A biological demulsifier and a technology for producing strains, applied in biochemical equipment and methods, methods based on microorganisms, methods using microorganisms, etc., to achieve the effect of low dosage concentration and shortened demulsification time

Inactive Publication Date: 2008-04-02
CHENGDU INST OF BIOLOGY CHINESE ACAD OF S
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no report on the research and application of biological demulsification for high-viscosity oilfield production fluid

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Embodiment one: the preparation of bacterial agent

[0028] 1. Bacteria: Bacillus subtilis Cohn, 1.1413, American H16-5

[0029] 2. Culture medium:

[0030] Culture medium includes:

[0031] NH 4 CL: 0.304%

[0032] K 2 HPO 4 .3H 2 O: 0.812%

[0033] MgSO 4 .7H 2 O: 0.02%

[0034] CaCl 2 .2H 2 O: 0.002%

[0035] pH6.0

[0036] Raw material oil for fermentation: liquid paraffin, dosage 3%

[0037] 3. Shake flask culture:

[0038] Two rings of each slant strain were placed in a 100ml Erlenmeyer flask, cultured on a shaker at 30°C and 180r / min for 48 hours, then transferred to a Erlenmeyer flask containing 500ml medium for 48 hours.

[0039] 4. Fermentation tank production

[0040] Add 5L of medium into the 10L stirred fermenter, and transfer a 500ml strain of the above-mentioned cultured large shake flask to the fermenter. The composite functional microbial agent is produced under different production conditions such as double fermentation, and the disch...

Embodiment 2

[0047] Example 2: Demulsification of oil-in-water produced fluid in Shengli Oilfield using fermented bacteria

[0048] Under the condition of 30-35 ℃, the production fluid of Gudao Oil Production Plant of Shengli Oilfield (crude oil viscosity 400mPa.s) was treated. In 10ml of oil-in-water oilfield emulsion, add the wet bacteria obtained by fermentation according to different proportions, shake it back and forth 200 times by hand, and let it stand for 10 minutes. Deals with demulsification effects. After several experiments, the following results were obtained: oil-water layer, the interface is black and white, neat, horizontal, in a line, the exfoliated water is colorless and transparent, and the oil content of the exuded water is 50-200ppm. The effective dosage of fermented wet bacteria is within 2‰.

Embodiment 3

[0049] Example 3: Demulsification test of oil-in-water produced fluid in Shengli Oilfield with fermented bacteria

[0050] Under the condition of 20-25 ℃, the production fluid of Gudao Oil Production Plant of Shengli Oilfield (crude oil viscosity 400mPa.s) was treated. In 10ml of oil-in-water oilfield emulsion, add the wet bacteria obtained by fermentation according to different proportions, shake it back and forth 200 times by hand, and let it stand for 10 minutes. Deals with demulsification effects. After several experiments, the following results were obtained: oil-water layer, the interface is black and white, neat, horizontal, in a line, the exfoliated water is colorless and transparent, and the oil content of the exuded water is 50-200ppm. The effective dosage of fermented wet bacteria is between 0.1-1‰.

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Abstract

A biologic demulsifier for the recovered oil-in-water liquid in oil field or the oil-in-water emulsion is prepared from bacillus and atoleine through fermenting and collecting the surficial thalluses of fermented liquid. Its advantages are high effect and high selectivity.

Description

1. Technical field: [0001] The invention relates to a demulsifier, in particular to a method for preparing a Bacillus and its fermented wet bacterium as a biological demulsifier and the application of the biological demulsifier to treat oil-in-water production fluid in oilfields and water-in-water produced by other industries. Biological treatment technology for oil-type emulsion to separate oil and water. 2. Background technology: [0002] At present, there are physical and chemical methods for demulsification of oilfield produced fluid abroad. Physical methods such as liquid film demulsification and electronic demulsification of O / W emulsion; chemical methods such as oil flotation recovery technology using acidification, heating, blasting and adding lime milk, adding flocculants and dissolved air flotation Two processes, air flotation and air flotation, are used to remove oil from oily wastewater, and chemical demulsifiers such as ethyleneamine / ethylene formamide copolyme...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N1/26E21B43/22B01D17/04C12R1/125
Inventor 李大平何晓红王晓酶田崇民彭世群
Owner CHENGDU INST OF BIOLOGY CHINESE ACAD OF S
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