Yeast expression high specic activity phytase gene obtained using chemical synthesis and molecular evolution

A technology for synthesizing genes and yeast expression vectors, applied in plant gene improvement, biochemical equipment and methods, genetic engineering, etc., can solve the problems of low expression of original strains, easy degradation of mutagenized strains, and high production costs
CN100398645CInactive Publication Date: 2008-07-02STAR LAKE BIOSCI CO INC ZHAOQING GUANGDONG
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
STAR LAKE BIOSCI CO INC ZHAOQING GUANGDONG
Publication Date
2008-07-02
Estimated Expiration
Not applicable · inactive patent

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Abstract

A phytase gene phyI with high specific activity suitable for yeast expression is prepared from synthetic gene through configuring mutant phytase gene library on prokaryotic expresstion plasmid, transferring to colibacillus, screening high-activity strains, and high-density fermenting.
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Description

technical field

[0001] The invention belongs to the field of microbial genetic engineering. Specifically, the acid phytase gene derived from Aspergillus niger is transformed by chemical synthesis method and in vitro recombination technology, so that it can be efficiently expressed in Pichia pastoris, and the expressed phytase is higher than that of The activity is improved and the structure is stable. Background technique

[0002] Phytase widely exists in plants, animals and microorganisms. Phytases produced by different species have great differences in specific activity, optimum reaction pH, heat resistance and other properties. Many microorganisms can produce phytase with high specific activity. In 1968, Shien et al. investigated 2000 strains from 68 soil samples and found that 21 of all 22 black mold strains could produce phytase. The first isolated and purified phytase is from Aspergillus terreus NO.9A-1, its optimal pH is 4.5, the optimal reaction temperature is 70°...

Claims

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