Medicine for treating and preventing tumour, and its prepn. method
A preparation and tumor technology, which is applied in the field of preparations for the prevention and treatment of tumors, can solve the problems of low tumor inhibition rate, complex components, and increase the difficulty of developing drugs, and achieve high anticancer activity, unique performance, and broad anticancer spectrum Effect
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Embodiment 1
[0050] Adopt the following photosynthetic bacteria culture medium, its formula is: sodium acetate 1500mg, CaCl 2 .2H 2 O 75mg, MgSO 4 .7H 2 O 200mg, EDTA 20mg, yeast extract 1000mg, K 2 HPO 4 1000mg, (NH 4 ) 2 SO 4 1500mg, KH 2 PO 4 700mg, FeSO 4 .7H 2 O 10mg, trace element solution 4ml, deionized water 1500ml. pH is 7.
[0051] Wherein the trace element solution composition and content are: H 3 BO 3 250mg, Na 2 MoO 4 .2H 2 O 75mg, CuSO 4 5mg, MnSO 4 .4H 2 O 200mg, ZnSO 4 .7H 2 O 25mg, deionized water 150ml.
[0052] Insert 50ml of Rhodopseudomonas palustris photosynthetic bacteria into 500ml of photosynthetic bacteria culture medium, under 1000Lux light, anaerobic condition, cultivate 10 days, as photosynthetic bacteria culture fluid.
[0053] The leafy stems of mistletoe Viscum coloratum (Kom.) Nakai were dried naturally and crushed. 125 g of pulverized mistletoe coarse powder is divided into three times with 1800 ml of 40% ethanol, each extraction is...
Embodiment 2
[0054] Embodiment 2: the preparation of photosynthetic bacteria culture fluid, photosynthetic bacteria bacterial classification adopts Rhodopseudomonas viridans and Rhodobacter sphaeroides mixed bacterial strain, other is with embodiment 1; , after natural air-drying, crushed, 125g of mistletoe coarse powder after crushing was extracted in 4 times with 3500ml of 95% ethanol, each time for 30 minutes, the filtrate was combined, and the ethanol in the extract was recovered under reduced pressure, concentrated to 150ml, and used in the culture medium Dilute to 1200ml, adjust the pH to 6, and sterilize at 121°C for 20 minutes to obtain the mistletoe medium mixture; inoculate 250ml of the photosynthetic bacteria culture medium into the above-mentioned mistletoe medium mixture, and cultivate for 10 days under 2000Lux light and anaerobic conditions. day, sterilized at 121°C for 40 minutes to make a sterilized preparation of photosynthetic bacteria transforming mistletoe.
Embodiment 3
[0055] Embodiment 3: the preparation of photosynthetic bacteria nutrient solution, photosynthetic bacteria bacterial classification adopts the bacterial strain that rhodospirillum rubrum, rhodopseudomonas green sulfur and marine rhodopseudomonas mix, and others are the same as embodiment 1; The leafy stems and branches of V.diospyrosicolum Hayata are naturally air-dried and pulverized. Divide 125g of the pulverized mistletoe coarse powder into 1500ml of 80% methanol for 2 times, extract for 4 hours each time, combine the filtrates, and recover the methanol in the extract under reduced pressure , concentrated to 200ml, diluted to 3000ml with culture medium, adjusted to pH 8, and sterilized at 121°C for 30 minutes to obtain a mistletoe medium mixture; inoculate 100ml of photosynthetic bacteria culture medium into the above-mentioned mistletoe medium mixture, and irradiate at 500Lux 1. Under anaerobic conditions, culture for 30 days, and then it will be made into a preparation of ...
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