Application of diarsenic trioxide in ion-exchange protein
A technology of arsenic trioxide and protein exchange, which is applied in the application field of arsenic trioxide as an agonist in ion exchange proteins, and can solve the problems of being easily reduced and easily oxidized, slow, and not yet seen.
Inactive Publication Date: 2008-11-19
SHANGHAI JIAOTONG UNIV SCHOOL OF MEDICINE
View PDF2 Cites 0 Cited by
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
[0004] Chemical formula: As 2 o 3 , Molecular weight: 197.84, Properties: Diarsenic trioxide has three states: amorphous, equiaxed, monoclinic crystals or colorless powder. They are easily reduced and oxidized. Melting point: Arsenic 275°C white arsenic Stone 313°C, solubility: 2g / 100ml. Arsenic trioxide has anti-tumor drug effect. In Chinese Patent Application No. CN02132755.6, a drug for preventing and treating restenosis of blood vessels is disclosed. The drug application of the drug. The drug has obvious effects of inhibiting the hyperplasia of the vascular intima at the damaged area and preventing restenosis. In the Chinese patent application number CN200410043871.8, a local slow-release chemotherapy drug of arsenic trioxide for the treatment of malignant brain tumors is disclosed. The The invention is composed of arsenic trioxide and polymer biodegradable polymer materials, and the anti-tumor arsenic trioxide is fused into a degradable polymer polymer by polymer compound method. Through the erosion and hydrolysis of the polymer, Release the drug slowly, continuously and stably and play a role. However, so far, there has been no report on the application of arsenic trioxide as an agonist in ion exchange proteins. Anion exchange protein 2 (AE2) is a member of the anion exchange protein family. Ubiquitous on the cell membrane, its function is to regulate the acid-base balance inside and outside the cell, cell volume and cell homeostasis. Scientists from all over the world are conducting research on its structure and function. During the research process, if there are agonists and inhibitors of it , will make the research on anion exchange protein 2 more in-depth and convenient. At present, there are only anion exchange protein 2 inhibitors on the market, and no agonists have been reported and developed.
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View moreImage
Smart Image Click on the blue labels to locate them in the text.
Smart ImageViewing Examples
Examples
Experimental program
Comparison scheme
Effect test
Embodiment 1
[0019] Cell Culture and Handling
[0020] Acute myeloid leukemia cell line NB4 (provided by Dr. Michael Lannotte21), acute monocytic leukemia cell line U937, acute myeloid leukemia cell line HL-60, Kasumi-1 and chronic myelogenous leukemia cell line K562 (from China Shanghai Institute of Biological Sciences Cell Bank) with 1640 culture medium containing 10% heated fetal bovine serum, at 37 ° C, containing 5% CO 2 The air and humid conditions were cultivated. During the experiment, the cells were 2-5×10 5 Inoculate at a density of 1 / ml, add the specified concentration of As 2 o 3 Or AE protein specific inhibitor DIDS (Sigma). Cell viability was detected by trypan-blue.
[0021] Western blotting
[0022] The prepared samples were analyzed with 9%-10% SDS-polyacrylamide, and electrotransferred onto nitrocellulose membranes (Amersham Bioscience, UK). Stained with 0.2% ponceau to ensure the presence of protein bands. After blocking with % TBS, respectively, with the antibody o...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More PUM
Login to View More Abstract
Description
technical field [0001] The present invention relates to a new application of arsenic trioxide, specifically the application of arsenic trioxide as an agonist in ion-exchange proteins. Background technique [0002] The structural formula of arsenic trioxide (Arsenic(III) oxide) is: [0003] [0004] Chemical formula: As 2 o 3 , Molecular weight: 197.84, Properties: Diarsenic trioxide has three states: amorphous, equiaxed, monoclinic crystals or colorless powder. They are easily reduced and oxidized. Melting point: Arsenic 275°C white arsenic Stone 313°C, solubility: 2g / 100ml. Arsenic trioxide has anti-tumor drug effect. In Chinese Patent Application No. CN02132755.6, a drug for preventing and treating restenosis of blood vessels is disclosed. The drug application of the drug. The drug has obvious effects of inhibiting the hyperplasia of the vascular intima at the damaged area and preventing restenosis. In the Chinese patent application number CN200410043871.8, a local s...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More Application Information
Patent Timeline
Login to View More Patent Type & Authority Patents(China)
IPC IPC(8): A61K33/36A61P43/00G01N33/50
Inventor 傅国辉
Owner SHANGHAI JIAOTONG UNIV SCHOOL OF MEDICINE