New biopesticide using gene from erwinia pyrifoliaeWT#3, novel pathogen that affects asian pear trees

A pathogen and gene technology, applied in the field of new biological pesticides, can solve the problem that pests are not recognized

Inactive Publication Date: 2009-06-03
PIONEER CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The general purpose of using biopesticides is to control pests by direct application of antagonistic microorganisms themselves to plants, but this is not considered very effective in pest control

Method used

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  • New biopesticide using gene from erwinia pyrifoliaeWT#3, novel pathogen that affects asian pear trees
  • New biopesticide using gene from erwinia pyrifoliaeWT#3, novel pathogen that affects asian pear trees
  • New biopesticide using gene from erwinia pyrifoliaeWT#3, novel pathogen that affects asian pear trees

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Embodiment 1: Isolation and identification of new bacterial strains

[0047] 1) Physiological / biochemical assays based on Schaad's experimental guidelines and Bergey's manual

[0048] In order to identify pathogenic bacteria from damaged parts infected by necrosis on Chunchon (Kangwon Province, Korea) orchard pears, based on Schaad's experimental guidance [Schaad, N.W. 1988. Preliminary identification of common genera. Ed. N.W. Schaad. American Phytopathological Society, Minnesot. PP.44-59] and Bergey's Handbook [Lelliott, R.A. and Dickey, R.S. 1984. Erwinia genus. In: Bergey Handbook of Systematic Bacteriology vol.1, pp.469-476, Williams and Willkins Co., Baltimore / London] performed different physiological and biochemical tests, as shown in Table 1 below.

[0049] Table 1

[0050]

[0051] Overall, the results of the analysis showed that this strain was superior to Erwiniapyrifoliae strain Ep16 in physiological assays (e.g., liquefaction of gelatin, motility in ...

Embodiment 2

[0099] Example 2: from Erwinia pyrifoliae WT # 3 that triggers plant hypersensitivity Properties of specific proteins and their coding genes

[0100] 1) Analysis of plant HR-induced genes from Erwinia pyrifoliaeWT # 3

[0101] To analyze genes encoding specific proteins that induce allergic responses in plants, the total DNA of Erwiniapyrifoliae WT #3 was incubated at 37°C to ensure its partial digestion by Sau3 AI. Then take 1 μl of DNA electrophoresis every 1 hour to check whether it is properly digested, and the result shows that the digestion of about 6 hours is the most suitable. The total DNA thus treated was prepared as insert DNA and ligated into the BamHI polylinker site of the cloning vector pLAFR3 using DNA ligase by incubation at 14°C for 12 hours. Pass the complete plasmid DNA ligated with insert DNA and vector through CaCl 2 Transformation method Transformation into E. coli (HB101). Then, the Escherichia coli strain was cultured on a Luria agar medium cont...

Embodiment 3

[0141] Embodiment 3: use the biological activity that plant HR inducible protein (Pioneer) carries out Research

[0142] 1) Control effect on cucumber powdery mildew (Sphaerotheca fuliginea)

[0143] To evaluate the control effect of Pioneer against powdery mildew, the gene encoding plant HR-induced protein was cloned into pKEP3 vector to purify the protein.

[0144] Cucumbers were grown using the conventional "rain-protecting" method prevalent on farms. Test material was administered based on a 3-repeat randomized complete block design. Cucumber stems and leaves were treated with HR-induced protein Pioneer at a dose of 20 μg / ml recommended by EDEN Bioscience.

[0145] EDEN Bioscience at the same dose As a control, the locally produced Fenarimol (chemical pesticide) was also applied according to the instructions for comparison. The processing method is as follows:

[0146] (1) 3 treatments: after the early stage of powdery mildew (Sphaerotheca fuliginea), use Pioneer...

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Abstract

The present invention relates to a biopesticide using a plant pathogen, and more particularly, to a biopesticide having an improved resistance against plant pathogens and an improved plant growth effect, which is superior to that of HrpN, a plant-senstive protein isolated from Erwinia amylovora ATCC 15580, by using a gene isolated from WT#3(Erwinia pyrifoliae WT#3)[KCCM 10283] thus enabling to be used as a fertilizer as well a biopesticide.

Description

technical field [0001] The present invention relates to a novel biopesticide utilizing a gene derived from a novel plant pathogen Erwinia pyrifoliae WT # 3 (KCCM 10283) isolated from Chunchon, Kangwon Province, Korea. This pathogen is only found in South Korea. This new biopesticide has more effective properties than HrpN, such as enhanced resistance to plant diseases, plant growth promotion and insect repellency. HrpN is derived from Erwinia amylovora (ATCC15580 T ), which does not exist in Korea. Thus, it can be used as a biopesticide and fertilizer that effectively controls plant diseases caused by pathogens and insects and promotes plant growth. Background technique [0002] Food shortage has emerged as one of the most serious problems facing people all over the world today. However, crop yields have been greatly reduced due to outbreaks of pests and diseases such as pathogens and insects. Currently, chemical pesticides are mainly used to prevent or control the sprea...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/00C12N15/31C12R1/18A01P7/04A01N63/50C05F11/08C05G3/60C07K14/27C12N1/21C12N5/10C12N15/09C12P21/00C12P21/02
CPCC12R1/18A01N65/00C07K14/27A01N63/50C12R2001/18C12N1/205A01N63/20C12N1/20
Inventor 林春根许长铉朴德焕裵后男赵埈模白秀真郑千淳崔信建
Owner PIONEER CO LTD
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