Method for obtaining transgenic grass for sheep
A transgenic, Leymus chinensis technology, applied in horticultural methods, other methods of inserting foreign genetic materials, botanical equipment and methods, etc., can solve the problem of unsystematic research on transgenic Leymus chinensis, Agrobacterium transformation limited to the host range, monocotyledonous plants Cultivate difficulties and other issues to achieve the effects of saving expenses, improving purity and quality, and reducing costs
Inactive Publication Date: 2009-06-10
INST OF BOTANY CHINESE ACAD OF SCI
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Problems solved by technology
[0004] At present, the protoplast transformation method is commonly used to introduce foreign DNA into recipient cells, but the protoplast culture of monocotyledonous plants is still very difficult, there are difficulties such as the protoplast regeneration system, and the transformation of Agrobacterium is limited to the host range
So far, there have been no reports of transgenic Leymus chinensis, and there is no systematic research on the method of obtaining transgenic Leymus chinensis capable of herbicide tolerance
Method used
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Embodiment 1
[0019] Embodiment 1. A method for obtaining transgenic Leymus chinensis tolerant to herbicides.
[0020] The process of the transformation system: vegetative propagation of raw materials→young panicle culture→obtain callus→subculture 3 times→transformation by gene gun→obtain and differentiate resistant callus→plant regeneration→obtain positive regenerated plants on rooting medium→ Transplanting of seedlings → molecular identification → further identification of herbicides. The specific method and process are as follows:
[0021] 1. Source and characteristics of plant materials
[0022] Leymus chinensis "W4" used in this example is produced in Hebei Province (W4 is a germplasm number in 1999), and "C3" is from Inner Mongolia (C3 is a germplasm number in 2000). The characteristics of the materials are listed in Table 1.
[0023] Table 1 Main traits and sources of the two genotypes
[0024]
[0025] 2. Medium
[0026] In this embodiment, the induction medium is supplement...
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Abstract
A method for preparing transgene grass for herbicide resistance includes: converting plasmid with pat gene into grass callus by gene gun method, culturing on screening culturing medium, screening resistant callus; transferring resistant callus into differential culturing medium and obtaining regenerative seed; inoculating regenerative seed to rooting culturing medium with screening agent to culture and obtaining transgene grass seed. It achieves low cost, high purity and quality.
Description
technical field [0001] The invention relates to a method for producing transgenic plants, in particular to a method for obtaining transgenic Leymus chinensis. Background technique [0002] Leymus chinensis is a monocotyledonous plant class, Poaceae, and Leymus genus, and is a perennial rhizome-type grass. Leymus chinensis shows strong ecological adaptability to different habitat conditions, which has great ecological significance for improving the ecological environment of grasslands in northern my country and salinized land in western China. Leymus chinensis is also an important forage resource, with high protein content, high nutritional value, good palatability, and resistance to trampling. It is a high-quality forage. However, due to the lack of scientific management of Leymus chinensis grasslands in my country, the grasslands are overloaded with grazing, and the biological yield and nutritional quality of grasslands have also dropped significantly. Large-scale grasslan...
Claims
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IPC IPC(8): C12N15/82C12N15/89C12N5/04C12N5/10A01H4/00
Inventor 刘公社
Owner INST OF BOTANY CHINESE ACAD OF SCI