Expression of soluble TRAIL protein

A soluble protein technology, applied in the field of bioactive protein expression, can solve the problems of high inclusion body content and low soluble TRAIL protein content, and achieve the effect of increasing expression and reducing the ratio
CN100500841CInactive Publication Date: 2009-06-17EAST CHINA UNIV OF SCI & TECH +1
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
EAST CHINA UNIV OF SCI & TECH
Publication Date
2009-06-17
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention opened a method to express the soluble TRAIL protein which includes cloning the extracellular gene of the TRAIL into the expression carrier with the PL promotor of the lambda bacteriophage; Transforming the cI+ host bacteria; expressing the recombinative TRAIL protein by the low-temperature induction. Also it can add the Zn2+ or the synthetic inhibiter of the prokaryotic protein into the medium in the inducing stage. The recombinative TRAIL protein is soluble, so it can simplify the isolation process.
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Description

technical field

[0001] The invention relates to a method for expressing biologically active proteins, in particular to a method for expressing tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) protein. technical background

[0002] Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) is one of the newly discovered TNF family members, also known as apoptotin 2ligand (Apo-2L). In 1996, Pitti first isolated and identified TRAIL and its induced apoptosis, and found that the full-length human TRAIL gene was 1769bp, located on chromosome 3q26. TRAIL protein is a type II transmembrane protein, consisting of 281 amino acids, with a molecular weight of 32.5kD and an isoelectric point of 7.63. functional parts. The biologically active form of TRAIL is a homotrimer, and there is a zinc ion binding site on the monomer interface (Sarah G et al., Biochem, 2000, 39:633-640).

[0003] The combination of the extracellular region of TRAIL and its specific receptor ...

Claims

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