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Chemical activator and its preparation and application

An activator, chemical technology, applied in the field of medicine, can solve the problems of toxic and side effects, use restrictions, and rarely reported anti-plant virus effects, etc.

Active Publication Date: 2009-08-19
KUNMING INST OF BOTANY - CHINESE ACAD OF SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to its toxic and side effects, the use is greatly restricted
At present, the chemical activators reported abroad are mainly used to resist pathogenic bacteria of crops, and rarely reported as anti-plant viruses.

Method used

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  • Chemical activator and its preparation and application
  • Chemical activator and its preparation and application
  • Chemical activator and its preparation and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Preparation of 3-acetonyl-3-hydroxyoxindole (3-acetonyl-3-hydroxyoxindole, AHO) (1):

[0021] (1) 17 kg of dry weight plant Strobilanthesis cusia whole herb was extracted with 10% acetone-methanol, and after removing the solvent under reduced pressure, an extract was obtained. , Methanol, and water were dissolved, and the solubles were prepared into corresponding 5-part extracts after removing the solvent, dissolved in DMSO to form a solution with a concentration of 10 mg / ml, and the anti-TMV activity of each part was measured by ELISA. The ethyl acetate extract was used as an effective component, and was repeatedly separated by various column chromatography methods to obtain a single anti-TMV active compound 3-acetonyl-3-hydroxyoxindole (3-acetonyl-3-hydroxyoxindole, AHO) ( 1) 17 mg.

[0022] Table 1 Proton spectrum and carbon spectrum data of compound (1)

[0023]

[0024] 3-acetonyl-3-hydroxyoxindole (3-acetonyl-3-hydroxyoxindole, AHO) (1)

[0025]

[0026] ...

Embodiment 2

[0032] The inhibitory rate test of compound (1) to TMV replication under different application conditions:

[0033] In order to explore the mechanism of action of the compound (1) against TMV, the present invention designs three drug testing methods: A) administering the drug first and then inoculating the virus, B) inoculating the virus first and then administering the drug, and C) mixing the drug and the virus for inoculation. The drug is divided into 5 concentrations, namely 100, 200, 300, 400, 500nM. Application method: apply the drug first and then inoculate the virus, or inoculate the virus first and then apply the drug, all are foliar application. The mixed inoculation of the drug and the virus is to mix different concentrations of compounds with TMV, place at 37°C for 15 minutes and then inoculate . Detection methods: scab detection, electron microscope detection, enzyme-linked immunoassay (ELISA) detection. The experimental results show that the virus inhibition rat...

Embodiment 3

[0037] Under optimal reagent conditions, the impact of different concentrations of compound (1) on TMV proliferation:

[0038] Different concentrations of compound (1) were evenly sprayed on the leaves of the systemic host tobacco K326, and then inoculated with TMV. Three days after the inoculation, the proliferation of the virus was detected by ELISA. The results showed that as the compound concentration increased, the virus content gradually decreased, and when the compound concentration was 500 nM (125 μg / ml), no virus could be detected (Table 3).

[0039] Table 3. Determination of plant viruses by enzyme-linked immunosorbent assay (ELISA)

[0040]

[0041] CK- is negative control, CK+ is positive control, greater than positive control is positive, negative control less than 2 times is negative.

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Abstract

3-acetonyl-3-hydroxyoxindole (3-acetonyl-3-hydroxyoxindole, AHO) (1), has a significant anti-tobacco mosaic virus (TMV) effect, studies have proved that AHO is by acting on the plant salicylic acid It is a new type of chemical activator and can be used to prevent and control plant diseases caused by plant viruses or pathogens.

Description

[0001] Field: [0002] The present invention relates to the field of medicines, more specifically, to 3-acetonyl-3-hydroxyoxindole (3-acetonyl-3-hydroxyoxindole, AHO) bulk drug and its preparation, its preparation method, and its use in the preparation and prevention of plant viruses Disease agents, anti-tobacco mosaic virus disease preparations, salicylic acid synthesis accelerators in plants, and plant systemic resistance activation preparations. Background technique: [0003] The research on chemical activators is considered to be one of the important development directions of new pesticides in the 21st century. In recent years, chemical activators such as INA and BTH have been developed abroad and used to activate the system of commercial crops to acquire resistance. These chemical activators are synthetic heterocyclic compounds that act on the upstream or downstream of the plant salicylic acid pathway. BTH has been registered and listed. However, due to its toxic and si...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N43/38A01N25/02A01P3/00
Inventor 郝小江李艳梅陈刚罗晓东张仲凯何红平
Owner KUNMING INST OF BOTANY - CHINESE ACAD OF SCI
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