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Extraction of natural melanin from myrtillin leaves

A technology of natural melanin and black rice leaves, applied in the direction of natural dyes, chemical instruments and methods, azo dyes, etc., can solve the problems affecting the extraction rate of melanin, and achieve the reduction of extraction mass transfer resistance, convenient operation, and good quality Effect

Inactive Publication Date: 2009-11-25
FUJIAN AGRI & FORESTRY UNIV
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Problems solved by technology

[0007] None of the above melanin extraction methods use enzyme preparations, which affects the extraction rate of melanin

Method used

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  • Extraction of natural melanin from myrtillin leaves

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Embodiment

[0023] Embodiment: A kind of method of extracting natural melanin from black rice leaf

[0024] The natural black rice leaves with a harvest period of 5-6 months are taken as raw materials, and the extraction method includes the following steps:

[0025] (1) Dry the fresh leaves of the black rice tree in an oven at 65°C, pulverize them with a high-speed tissue grinder, and sieve them through a 40-mesh sieve to obtain dry powder of the black rice tree leaves for use.

[0026] (2) Take 180ml of 0.1mol / L citric acid solution and 320ml of 0.2mol / L disodium hydrogen phosphate solution, mix and dilute to 1000ml to prepare a buffer solution.

[0027] (3) Take 250ml of buffer solution, add 0.05g of pectinase and 0.075g of cellulase, stir to dissolve completely, and form a uniform enzyme solution.

[0028] (4) Add 10 g of dry powder of black rice leaves to the above-mentioned enzyme liquid.

[0029] (5) After stirring evenly, keep it in a constant temperature water bath at 50°C in th...

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Abstract

The invention discloses a method for extracting natural melanin from the leaves of black rice plant, which uses the natural plant black rice leaf as the main raw material, and uses enzyme preparation combined with a solvent to extract the natural melanin. The preparation method comprises the following steps: material preparation, preparation of buffer solution, preparation of enzyme solution, feeding, enzymolysis, extraction and finished product. The natural melanin prepared by the method of the present invention has good product safety and good quality, and the dosage of enzyme preparation is less, and the extraction rate of melanin is 30-40% higher than that of the alcohol extraction method, and the process is simple, easy to operate, high in efficiency, and suitable for industrialization Production.

Description

Technical field [0001] The invention relates to a method for extracting natural pigment, in particular to a method for extracting natural melanin from the leaves of black rice plant. Background technique [0002] Melanin in plants has various pharmacological activities such as promoting resynthesis of retinoid, Vp-like function, improving blood microcirculation, anti-ulcer, anti-inflammation, and anti-aging. It includes various pigment components such as anthocyanins and flavonoid pigments, and the extraction of melanin is mainly by solvent extraction. [0003] "Food and Fermentation Industry" published the article "Extraction and Separation and Purification of Flavonoid Pigments from the Leaves of Wufan" by Wang Li and Yao Yuanhui in Volume 30, Issue 9, 2004, and established the best extraction conditions for flavonoid pigments: extraction times 2 times , ethanol volume fraction 60%, temperature 60°C, solid-liquid ratio 1g: 35ml. [0004] "Chinese Wild Plant Resources" pu...

Claims

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Application Information

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IPC IPC(8): C09B61/00
Inventor 陈绍军余清
Owner FUJIAN AGRI & FORESTRY UNIV
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