Yeast glucans extraction process

A technology of yeast glucan and extraction process, applied in the direction of fermentation, microorganism-based methods, microorganisms, etc., can solve the problems of large waste liquid, high cost, complex process, etc., reduce the amount of acid and alkali, increase added value, and extract The effect of fewer processes

Inactive Publication Date: 2007-08-08
TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

The protein content of acid-base extraction of glucan is low, but the yield is low and the process is complicated, the cost is hig

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0011] Example 1: A process for extracting glucan from yeast cell walls using high temperature combined with enzyme preparation, which is achieved through the following steps:

[0012] (1) High temperature extraction: use ordinary yeast cell wall as raw material, add water to make a suspension at 1:3, heat to 105°C, after 1 hour, cool to 40°C, dilute the remaining insoluble matter with water, centrifuge, and wash twice with water.

[0013] (2) Separation: Take the precipitate after centrifugation, dilute it with water at 1:15, heat it to 30℃, adjust the pH to 9, add alkaline protease, the amount of which is 0.2% of the precipitate, after enzymatic hydrolysis for 2 hours, medium And, the obtained insoluble matter is centrifuged and washed with water.

[0014] (3) Removal of impurities: the precipitate washed with water after protease treatment is treated with excess acetone, filtered, and the remainder is washed with acetone three times.

[0015] (4) Drying: The obtained precipitat...

Example Embodiment

[0017] Example 2: A process for extracting glucans from yeast cell walls using high temperature combined with enzyme preparations, which is achieved through the following steps:

[0018] (1) High-temperature extraction: use ordinary yeast cell wall as raw material and add water to make a suspension at a ratio of 1:8, heat to 150°C, after 6 hours, cool to 50°C, dilute the remaining insoluble matter with water, centrifuge, and wash 3 times with water.

[0019] (2) Separation: Take the precipitate after centrifugation and dilute it with water at 1:25, heat it to 40°C, adjust the pH to 11, add papain, the amount of which is 0.4% of the precipitate, and neutralize it after enzymatic hydrolysis for 6 hours. The obtained insoluble matter was centrifuged and washed with water.

[0020] (3) Removal of impurities: the precipitate washed with water after protease treatment is treated with excess acetone, filtered, and the remainder is washed with acetone three times.

[0021] (4) Drying: The...

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PUM

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Abstract

The invention discloses a manufacturing technique of yeast gluglucosan to decompose yeast cellar wall conjugase under high temperature, which compriss the following steps: adopting yeast cell wall as raw material; adding water with weight rate at 1:3-1:8 to make suspension; heating to 105-150 deg.c for 1-6h; cooling; diluting; centrifuging; washing for 2-3 times; removing sediment; adding water with rate at 1;15-1:25; heating to 30-40 deg.c; adjusting pH value to 9-11; stirring; adding proteinase; enzymolyzing 2-6h; neutralizing; centrifuging; washing; disposing through excessive ketone; filtering; washing residual for three times through ketone; freezing sediment at -70 deg.c for 22h; freezing sediment at -60 deg.c for 6-10h; obtaining the product.

Description

Technical field: [0001] The invention relates to a process for extracting yeast glucan by combining high temperature and enzyme preparation, in particular to a process for extracting glucan in yeast cell wall by using high temperature and enzyme preparation. Background technique: [0002] Yeast is an important industrial microorganism. my country is rich in yeast resources. In recent years, the market demand for yeast product diversification has increased, which has accelerated the development of my country's yeast processing industry. However, yeast processing will produce a large amount of yeast cell walls, which contain about 30% β-D glucan. The dextran extracted from the yeast cell wall can activate macrophages, neutrophils, etc., so it can increase the content of leukin, cytokinin and special antibodies, and fully stimulate the body's immune system. At present, the extraction methods of yeast glucan include acid-base method and ultrasonic treatment method. The acid-ba...

Claims

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Application Information

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IPC IPC(8): C12P19/04C12R1/85
Inventor 杜丽平肖冬光祁业明陈叶福郭学武
Owner TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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