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Process of separating and purifying natural theanine

A technology for separation and purification of theanine, which is applied in the field of separation and purification of natural theanine, can solve problems such as not being comprehensively utilized, and achieve good regeneration performance

Inactive Publication Date: 2007-08-22
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are many domestic manufacturers who use low-grade tea to extract tea polyphenols. The wastewater discharged after extracting tea polyphenols contains a certain amount of theanine, which is basically not comprehensively utilized at present.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] (1) Ultrafiltration process: After the tea polyphenol industrial waste liquid is microfiltered by 0.2μm microfiltration membrane, polysulfone ultrafiltration membrane with a molecular weight cut-off of 6KD is selected, and the operating pressure is 0.08-0.10Mpa and the temperature is 30-40℃. Perform ultrafiltration to obtain ultrafiltration permeate.

[0021] (2) Macroporous adsorption resin decolorization: Ultrafiltration permeate passes through a chromatography column equipped with D301-G macroporous adsorption resin at room temperature at 4 column bed volume / hour flow rate, and the equivalent processing capacity is 16 times the column bed. Stop loading when the volume is reached, top wash with 2 times the column bed volume of deionized water, and combine the effluent before the saturation point and the top wash to obtain a clear liquid.

[0022] (3) Cation exchange resin adsorption: replace the 001×7 resin with hydrogen form and load the column, concentrate the decoloriz...

Embodiment 2

[0028] (1) The ultrafiltration process is the same as in Example 1.

[0029] (2) Decolorization of macroporous adsorption resin: Stop loading when the processing capacity is 18 times the column bed volume, top wash with 3 times the column bed volume of deionized water, and combine the effluent before the saturation point and the top wash to obtain a clear solution. The remaining conditions are the same as in Example 1.

[0030] (3) Cation exchange resin adsorption: Concentrate the decolorized clear liquid to a theanine concentration of 2.5mg / ml, adjust the pH to 3.2 with hydrochloric acid, use a flow rate of 2.0 bed volume / hour on the column, and load 10 times the sample volume The column bed volume. The remaining conditions are the same as in Example 1.

[0031] (4) Ammonia water elution: After adsorption equilibrium, the column is washed with 3 times the bed volume of deionized water, and the flow rate is 3.0 bed volume / hour. Then eluted with a pH 11.3 aqueous ammonia solution, ...

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Abstract

The process of separating and purifying natural theanine belongs to the field of deep tea processing technology. The present invention uses the industrial waste liquid from tea polyphenol production as material to produce natural theanine through the steps of ultrafiltering, macroporous resin decolorizing, cationic exchange resin adsorption, ammonia water elution, microcrystal cellulose column chromatography and anhydrous alcohol crystallization. The present invention has theanine product purity over 95 % and total theanine recovering rate over 60 %, and is environment friendly.

Description

Technical field [0001] A method for separating and purifying natural theanine belongs to the technical field of tea deep processing. Background technique [0002] Theanine is a unique non-protein amino acid in tea, and it is also one of the taste substances of tea. It accounts for about 50% to 60% of the total amount of amino acids in tea. Theanine is an amide compound, chemically named: N-B Group-γ-glutamine. Studies have shown that theanine has physiological functions such as affecting neurotransmitter secretion in the brain, antagonizing caffeine-induced excitement, relieving tension, lowering blood pressure, anti-fatigue, and assisting in inhibiting tumors. At present, it is mainly used in medicines and food additives, and the demand for theanine is also increasing. For the extraction and synthesis of theanine, which is more suitable for industrial scale-up and other issues still need to be further studied. Scientists at home and abroad have researched and developed a variety...

Claims

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Application Information

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IPC IPC(8): C07C231/24C07C237/22
Inventor 王洪新朱松
Owner JIANGNAN UNIV
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