Iron bacterium testing fluid and simple testing tube

A technology of iron bacteria and test bottle, which is applied in the field of iron bacteria test solution and its test bottle in water or other substances, which can solve the problems of high quality requirements for test environment and operators, inaccurate test results, cumbersome operating procedures, etc. Achieve accurate and reliable test results, shorten test time, and reduce labor intensity

Inactive Publication Date: 2010-10-06
白莉
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The multi-tube fermentation method is not only cumbersome, complicated, and time-consuming, but also has extremely high requirements for the quality of the test environment and operators, from sampling technology, laboratory conditions, instruments and equipment, container reagent quality, operator skills, to quality Control and other monitoring processes and control procedures have strict regulations and requirements
In addition, the complicated test process can easily produce large errors, resulting in inaccurate test results

Method used

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  • Iron bacterium testing fluid and simple testing tube
  • Iron bacterium testing fluid and simple testing tube

Examples

Experimental program
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Effect test

Embodiment 1

[0023] Embodiment one: see figure 1 In the figure, 1 is an aluminum skin cover, 2 is a rubber stopper, 3 is a glass bottle cavity with equal cross-section, and 4 is a test solution for iron bacteria. h 1 is the height of the iron bacteria test solution, H 2 Empty height for the equal section of the test bottle. In the present embodiment, the test bottle is a colorless transparent oral liquid bottle of 15mL, and the height H of the test solution is 1 is 6cm, and the height of the space is H 2 It is 4cm.

[0024] The formula of the above test solution is: 0.05% of magnesium sulfate, 0.01% of calcium chloride, 0.5% of ferric ammonium citrate, 0.03% of dipotassium hydrogen phosphate, 0.01% of sodium nitrate, and 97.9% of distilled water. After the test solution is prepared, pack 9.0mL per bottle into a 15mL colorless transparent oral solution bottle, and then sterilize it by autoclaving for 15 minutes.

[0025] When using, use a sterile syringe to draw 1.0mL water quality sa...

Embodiment 2

[0026] Embodiment two: the present embodiment is basically the same as embodiment one, and the difference is that the test bottle is a colorless transparent vial of 8mL, and the height H of the test solution is 1 is 4.5cm, and the height of the space is H 2 It is 3.5cm.

[0027] The formula of the test solution is: 0.3% of magnesium sulfate, 0.1% of calcium chloride, 1.5% of ferric ammonium citrate, 0.1% of dipotassium hydrogen phosphate, 0.1% of sodium nitrate, and the balance is distilled water. After the above-mentioned test solutions are prepared, pack 4.5 mL per bottle into 8 mL colorless transparent vials, and sterilize under high pressure for 15 minutes.

[0028] During use, draw 0.5 milliliters of water samples with a sterile syringe and inject them into the test bottle, and the other steps are the same as in Example 1.

Embodiment 3

[0029] Embodiment three: the present embodiment is basically the same as embodiment one, and the difference is that the test bottle is a colorless transparent round-bottomed glass bottle of 10mL, and the height H of the test solution is 1 is 3.0cm, and the height of the space is H 2 It is 0.9cm.

[0030] The formula of the above test solution is: 0.1% of magnesium sulfate, 0.02% of calcium chloride, 1.0% of ferric ammonium citrate, 0.05% of dipotassium hydrogen phosphate, 0.05% of sodium nitrate, and the balance is distilled water. After the above test solution is prepared, pack 4.5 ml of each bottle in a 10 mL test bottle and autoclave for 15 minutes.

[0031] The method of use is the same as in Example 2, and will not be repeated.

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Abstract

A method for testing microbe, its test liquid and tester are disclosed. The ferrite bacterium test liquid consists of sulfate 0.05-0.3%, chloride 0.01-0.1%, ferric ammonium 0.5-1.5%, phosphate 0.03-0.1%, nitrate 0.01-0.1% and distilled water 97.9-99.4%. Height H1 of test liquid: constant-section space height H2=1:0.3-0.8. It's fast, convenient and correct and has long storage time. It can be usedfor water and sludge deposits.

Description

technical field [0001] The invention relates to a method for testing microorganisms in water and sludge deposits and a test device thereof, in particular to a test liquid for iron bacteria in water or other substances and a test bottle thereof. Background technique [0002] Iron bacteria widely exist in water and various water supply and drainage pipes. Their growth, metabolism and reproduction can easily cause corrosion of metal materials, destructive deterioration and deterioration, and lead to corrosion of pipes and ship shells, resulting in serious economic losses. [0003] At present, there is no national standard method for the determination of iron bacteria. The traditional determination method is the multi-tube fermentation method. The general operating procedure is: ① Collect water samples → ② Prepare sterile water → ③ Sterilize containers and reagents → ④ Prepare medium → ⑤Dilution and inoculation of water samples → ⑥ Bacterial culture → ⑦ Counting and reporting. I...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/04C12M1/34
Inventor 王济昌白莉宋毛平李树华靳文义
Owner 白莉
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