Method of extracting matrine by ultrasound wave method

An ultrasonic method and a technology of matrine, applied in the direction of medical formula, medical preparations containing active ingredients, plant raw materials, etc., can solve the problems of low extraction rate, long extraction time, long extraction line of matrine, etc., and achieve improved The effect of extraction rate, shortening extraction time, and high practical promotion value

Inactive Publication Date: 2007-10-24
NORTHEAST AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a more suitable process route for extracting matrine, which solves the shortcomings of long matrine extraction line,

Method used

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  • Method of extracting matrine by ultrasound wave method
  • Method of extracting matrine by ultrasound wave method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment approach 1

[0027] Accurately weigh 20g of Sophora flavescens powder (through a 40 mesh sieve), use 70% ethanol as solvent, the solid-liquid ratio of Sophora flavescens powder and solvent (solvent volume ml / massage of flavescens powder mass g) is 7, and the ultrasonic power is controlled at 300~ 700W ultrasonic extraction for 40min, with detection conditions as chromatographic column model: C 18 column; detection wavelength: 220nm; mobile phase: methanol: water: triethylamine (71.80: 27.85: 0.35); flow rate: 1.0ml / min; The comparison of extraction rates of matrine in the Pharmacopoeia is shown in Table 2. Add acid to remove tanning and adjust the pH to 3. After cationic resin, silica gel, Al 2 o 3 Exchange, elute with chloroform:methanol ratio of 9:1, concentrate, and recrystallize to obtain pure matrine, the purity of which is greater than 98% by liquid chromatography. See Figure 2 for the liquid chromatogram.

[0028] Ultrasonic frequency (W)

Embodiment approach 2

[0030] Accurately weigh 20g of Sophora flavescens powder (pass through a 40 mesh sieve), use 70% ethanol as solvent respectively, control the ultrasonic power at 600W, and the solid-liquid ratio of Sophora flavescens powder and solvent (solvent volume ml / matrix flavescens powder mass g) is 5 ~13 Ultrasonic extraction for 40min, with detection conditions as chromatographic column model: C 18 column; detection wavelength: 220nm; mobile phase: methanol: water: triethylamine (71.80: 27.85: 0.35); flow rate: 1.0ml / min; The comparison of extraction rates of matrine in the Pharmacopoeia is shown in Table 3. Add acid to remove tanning and adjust the pH to 3. After cationic resin, silica gel, Al 2 o 3Exchange, elute with chloroform:methanol ratio of 9:1, concentrate, and recrystallize to obtain pure matrine, the purity of which is greater than 98% by liquid chromatography.

[0031] Ratio of solid to liquid (ml / g)

Embodiment approach 3

[0033] Accurately weigh 20g of Sophora flavescens powder (through a 40-mesh sieve), set the solid-liquid ratio of Sophora flavescens powder to solvent (volume of solvent in ml / mass of flavescens powder in g) as 11, control the ultrasonic power at 500W, and pH 2 to 6 hydrochloric acid Ultrasonic extraction of solvent for 30 minutes, with detection conditions as chromatographic column model: C 18 column; detection wavelength: 220nm; mobile phase: methanol: water: triethylamine (71.80: 27.85: 0.35); flow rate: 1.0ml / min; The comparison of extraction rates of matrine in the Pharmacopoeia is shown in Table 4. Add acid to remove tanning and adjust the pH to 3. After cationic resin, silica gel, Al 2 o 3 Exchange, elute with chloroform:methanol ratio of 9:1, concentrate, and recrystallize to obtain pure matrine, the purity of which is greater than 98% by liquid chromatography.

[0034] pH of HCl

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Abstract

The invention discloses an extracting method of matrine through ultrasonic method, which comprises the following steps: setting the bulk rate of flavescent sophora and solvent at 1:3-15; disposing the composite liquid through ultrasound at 200-1000W below 60 deg. c; decompressing; filtering; adding acid to adjust pH value; adding organic solvent to extract the filtrate; separating the condensed evaporated organic solvent through column chromatography; crystallizing; recrystallizing; obtaining the monomer of the product. The invention avoids sophocarpidine from damaging due to high temperature, which saves time and labor effectively.

Description

technical field [0001] The present invention is an ultrasonic method for extracting matrine, which belongs to the field of natural product extraction and purification. It uses a certain intensity of ultrasound to act on alcohol or acid or water as a solvent system to extract matrine or other plants containing matrine. Methods. Background technique [0002] The contents of matrine, oxymatrine, sophocarpine, sophoridine and oxysophocarpine in Sophora flavescens account for 90% of the total alkaloid content in Sophora flavescens. The pharmacological and pharmacodynamic tests of matrine in humans and other animals show that its drug development and application prospects are very broad, but the current extraction technology for matrine is not perfect, and most of them still use traditional methods such as decoction and percolation. It is time-consuming and laborious, and the extraction rate is not high, and there are also problems such as difficult separation and low purity. Ho...

Claims

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Application Information

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IPC IPC(8): C07D471/22C07D455/00A61K36/489
Inventor 叶非白靖文付颖张鑫
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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