Retinene membrane protein and groundmass composite material, and method of producing the same
A composite material, retinal technology, applied in the field of retinal membrane protein and matrix composite materials and their preparation, can solve problems such as destruction, data error, unfavorable preservation and the like
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Embodiment 1
[0018] Bacteriorhodopsin-gelatin cross-linked composite film
[0019] Weigh 1g of gelatin particles, add it to 10mL water, stir in a hot water bath at 60°C until completely dissolved, and obtain a gelatin solution with a concentration of 10% (wt). 1 m 1 The bacteriorhodopsin protein extracted from the lyophilized protein was freeze-dried into a dry powder, weighed 40mg of the dry protein powder and suspended it in 0.5mL water, and ultrasonically dispersed it on a YJ92-II ultrasonic cell pulverizer with a power of 100w, an ultrasonic time of 5s, and an interval of 30s, and ultrasonicated 15 times. Take 0.5 mL of 10% gelatin solution, add it to the protein suspension, stir and mix in a hot water bath, and then ultrasonically disperse again, with a power of 100w, ultrasonic time of 5s, interval of 30s, and ultrasonication 15 times to obtain a clear and transparent mixed solution. Filter the mixed solution through an ester microporous membrane, place it in a hot water bath, weigh...
Embodiment 2
[0021] Bacteriorhodopsin D96N Mutant-Gelatin Crosslinked Composite Film
[0022] Weigh 2 g of gelatin particles, add them to 10 mL of water, stir in a hot water bath at 60°C until completely dissolved, and obtain a gelatin solution with a concentration of 20% (wt), freeze-dry the bacteriorhodopsin D96N mutant protein into a dry powder, and weigh the protein dry powder Suspend 5mg in 0.5mL water, ultrasonically disperse on a YJ92-II ultrasonic cell pulverizer, power 150w, ultrasonic time 4s, interval 40s, ultrasonication 10 times, absorb 0.5mL 20% gelatin solution, add to the protein suspension, put in a hot water bath Stir and mix, ultrasonically disperse again, power 150w, ultrasonic time 3s, interval 50s, ultrasonic 10 times to obtain a clear and transparent mixed solution, filter the mixed solution with a 5 μm cellulose ester microporous membrane while it is hot, place it in a hot water bath, weigh Take 10 mg of EDC, dissolve it with a small amount of water, add it to the m...
Embodiment 3
[0024] Composite Membrane Containing Archaeordopsin 4
[0025] Weigh 3 g of acrylamide (acr for short), 8 mg of N,N-methylene-bisacrylamide (Bis for short), dissolve them in 10 mL of water to obtain a monomer solution with a concentration of 30% (wt), store at 4°C, The ancient rhodopsin 4 protein extracted from strain xz515 was freeze-dried into dry powder, weighed 15mg of protein dry powder, added to 1mL of 30% acrylamide monomer solution, stirred and mixed, and ultrasonically dispersed on a YJ92-II ultrasonic cell pulverizer, power 200w, ultrasonic time 3s, interval 50s, ultrasonication 5 times to obtain a clear and transparent mixed solution, filter the mixed solution with a 5μm cellulose ester microporous membrane, weigh 8mg of EDC, 1mg of ammonium persulfate (ammonium persulfate referred to as AP), and use a small amount of water After dissolving, add it to the mixture, stir gently to avoid air bubbles, absorb an appropriate amount of liquid, spread it in a circular membr...
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