Grass spirillum with aerobic denitrification and method for processing wastewater

A technology for aerobic denitrification and wastewater treatment, applied in aerobic process treatment, chemical instruments and methods, sustainable biological treatment, etc., can solve problems such as anoxic denitrification

Inactive Publication Date: 2008-02-27
BEIJING TECHNOLOGY AND BUSINESS UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This strain has the characteristics of denitrification under aerobic conditions. It can be inoculated in conventional aerobic nitrification sludge to realize simultaneous nitrification and denitrification of

Method used

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  • Grass spirillum with aerobic denitrification and method for processing wastewater
  • Grass spirillum with aerobic denitrification and method for processing wastewater

Examples

Experimental program
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Effect test

Embodiment 1

[0014] Embodiment 1: Determination method of aerobic denitrification performance of Herbaspirillum huttiense CGMCC No.1799 strain

[0015] In order to prevent the introduction of miscellaneous bacteria into the simulated wastewater, both the simulated nitrate wastewater I and the simulated nitrate wastewater II were inactivated in a high-temperature sterilization pot at 0.1 MPa for 20 minutes. Get 5ml of sterilized nitrate simulated waste water I and put it into a test tube, insert the Herbaspirillum huttiense CGMCC No.1799 bacterial strain sample preserved in liquid paraffin seal. The test tube was placed in an air bath constant temperature shaker, the constant temperature was maintained at 30°C, and the shaker speed was 160r / min to activate the strain. After activating for 12 hours, inoculate 10% of the inoculum into a 250ml Erlenmeyer flask filled with 200ml nitrate simulated waste water II to measure denitrification and denitrification performance. In order to ensure the ...

Embodiment 2-10

[0019] Example 2-10: Determination of optimal denitrification conditions for Herbaspirillum huttiense CGMCCNo.1799 strain under different experimental conditions and ranges

[0020]The present invention carries out 9 waste water treatment tests, preferably the aerobic denitrification reaction conditions of Herbaspirillum huttiense WXZ-14CGMCC No.1799 bacterial strain.

[0021] The four reaction factors of wastewater aerobic denitrification are: dissolved oxygen concentration, which is realized by controlling different rotational speeds rpm. The three levels of rotational speed are 60, 100 and 160 rpm, and the corresponding dissolved oxygen concentrations are 1.8-3.5 mg / L ( 24~46% saturated dissolved oxygen), 4.2~5.6mg / L (55~74% saturated dissolved oxygen) and 6~7.2mg / L (79~95% saturated dissolved oxygen); the three levels of nitro nitrogen concentration are respectively The three levels of carbon-nitrogen ratio are 2, 6 and 10 respectively; the three levels of pH are 6.5, 7.0 ...

Embodiment 11

[0026] Embodiment 11: PCR amplification and sequence determination of the 16SrRNA gene of Herbaspirillum huttiense CGMCC No.1799

[0027] Inoculate Herbaspirillum huttiense CGMCC No.1799 into 100 μL of double-distilled water, boil in a boiling water bath for 5 minutes, and perform colony PCR amplification. The primers used for the 16SrRNA PCR reaction were a pair of universal primers, that is, the forward primer was f27 (5'-AGAGTTGATCCTGGCTCAG-3') and the reverse primer was r1492 (5'-GGTTACCTTGTTACGA CTT-3'). Primers were synthesized by Beijing Huada Zhongsheng Technology Development Co., Ltd. PCR reaction system (50 μL): 5 μL of 10×Buffer, 4 μL of dNTPs, 1 μL of primers f27 and r1492, 39 μL of double distilled water, centrifuged and mixed, and then added 0.5 μL of DNA template and 0.5 μL of Taq enzyme. The PCR reaction program included: (1) pre-denaturation at 94°C for 5 min; (2) denaturation at 94°C for 1 min; (3) annealing at 50°C for 1 min; (4) extension at 72°C for 2 min...

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Abstract

The invention discloses a WXZ-14 CGMCC No. 1799 strain of Herbaspirillum huttiense with aerobic denitrifying property and application to dispose and denitrify waste water, which is characterized by the following: displaying strong tolerant degree for the dissolved oxygen; reducing nitrate and nitrite at obvious speed under 10-98% saturated oxygen dissolving condition; inoculating the strain in the active sludge to do enriching culture; realizing the goal of aerobic denitrification.

Description

technical field [0001] The invention relates to a method for biological denitrification treatment of wastewater by adopting Herbaspirillum huttiense WXZ-14CGMCC No.1799 strain under aerobic conditions. The strain has strong tolerance to dissolved oxygen, and can use nitrate and nitrite as electron acceptors under the condition of 10-98% saturated dissolved oxygen. Nitrate and nitrite in sewage can be reduced to gaseous products in an aerobic environment to denitrify wastewater. Background technique [0002] Among the various denitrification methods that have been applied so far, biological denitrification is still the main method in wastewater denitrification treatment. The traditional wastewater biological denitrification treatment process is: the organic nitrogen compounds in the sewage are first converted into ammonia nitrogen in the biochemical process, and the ammonia nitrogen is converted into nitrate and nitrite by autotrophic nitrifying bacteria in the nitrification...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/02C02F3/34
CPCY02W10/10
Inventor 汪苹项慕飞翟茜龙雯柯国华杨志
Owner BEIJING TECHNOLOGY AND BUSINESS UNIVERSITY
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