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Method for high efficiency extracting momordica polysaccharide from balsam pear

A kind of bitter gourd polysaccharide, bitter gourd technology, applied in application, food preparation, food science and other directions, can solve the problems of weakened physical and chemical properties and physiological functions, low product yield and purity, unsuitable for industrial application, etc., to avoid heating and high energy The effect of consumption, low production cost, simplified production process and cost

Active Publication Date: 2008-03-26
GUANGZHOU JINYANG COSMETICS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Some traditional methods of extracting bitter gourd polysaccharides, such as hot water extraction, dilute salt, dilute alkali solution extraction, enzymatic hydrolysis extraction, etc., most of the products obtained have low yield and purity, and are time-consuming and energy-consuming. Weakness or even loss of physical and chemical properties and physiological functions
And other studies have obtained that although the purity of the product is high, the process is complicated, the cost is high, and the yield is low, which is not suitable for industrial application, and the direct product of bitter melon polysaccharide is also rare in the market.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] The first step is to weigh 10 kg of fresh bitter gourd, slice, vacuum dry, and crush to obtain bitter gourd powder; extract the bitter gourd powder with 85% ethanol at 60°C for 2 times, each time for 6 hours, to obtain purified bitter gourd powder; the filtrate is distilled to recover ethanol and bitter gourd powder The raw material that is used for subsequent steps after drying; The volume-to-mass ratio of ethanol and bitter gourd powder is 4: 1ml / g;

[0025] In the second step, 9 g of the bitter melon powder in the first step is added to 100 ml of distilled water, and treated with unikzyme C20 cellulase provided by Guangzhou Yulibao Company for 10 minutes. The mass percent concentration of cellulase in the purified bitter melon powder distilled water is 0.2%. Filter and retain the filter residue, then add distilled water to it according to the mass ratio of 1:30, treat with 700w Sanle WP650 (or other microwave equipment) microwave for 5min, then heat in a water bath at...

Embodiment 2

[0029] The first step is to weigh 10kg of fresh bitter gourd, slice, vacuum dry, and crush to obtain bitter gourd powder; extract the bitter gourd powder with 85% ethanol at 60°C for 2 times, each time for 5 hours, to obtain purified bitter gourd powder; the filtrate is distilled to recover ethanol, and bitter gourd powder The raw material that is used for subsequent steps after drying; The volume-to-mass ratio of ethanol and bitter gourd powder is 4: 1ml / g;

[0030] In the second step, take 10 g of the bitter melon powder in the first step and add 100 ml of distilled water, and treat it with unikzyme C20 cellulase for 12 minutes. Add distilled water to it at a ratio of 1:25, 1000w microwave extraction for 3min, then heat in a water bath at 60°C for 3.5h and filter to obtain the filtrate;

[0031] Step 3 Add unikneutral L neutral protease with a concentration of 0.5wt% to the solution obtained in the second step and treat it at 45°C for 16 minutes, centrifuge to take the super...

Embodiment 3

[0034] The first step is to weigh 10 kg of fresh bitter gourd, slice, vacuum dry, and crush to obtain bitter gourd powder; extract the bitter gourd powder with 85% ethanol at 60°C for 2 times, each time for 5.5 hours, to obtain purified bitter gourd powder; the filtrate is distilled to recover ethanol, and the bitter gourd The raw material that powder is used for subsequent steps after drying; The volume mass ratio of ethanol and bitter gourd powder is 4: 1ml / g;

[0035] The second step is to take 9.5g of the bitter melon powder in the first step and add 1000l of distilled water, and treat it with unikzyme C20 cellulase for 11min. The mass percentage concentration of the cellulase in the distilled water of the purified bitter melon powder is 0.4%. Distilled water was added to it at a mass ratio of 1:28, microwaved at 700w for 4.5min, then heated in a water bath at 58°C for 3.8h, and filtered to obtain the filtrate;

[0036] Step 3 Add unikneutral L neutral protease with a conc...

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Abstract

The present invention discloses a method for extracting balsam pear polysaccharide from balsam pear. Said method includes the following steps: slicing fresh balsam pear raw material, vacuum drying, grinding the dried balsame pear slices to obtain balsam pear powder, using ethyl alcohol to make extraction, filtering and drying so as to obtain the balsam pear powder which can be used as raw material for subsequent process; adding distilled water into said balsame pear powder, using cellulase whose mass percentage concentration is 0.2-0.6% to make treatment for 10-12 min, filtering to obtain residue, then adding distilled water according to the mass ratio of 1:25-30, using wicrowave with 700W-1000W to make treatment for 3-5 min, heating for 3.5-4h in water bath with 55-60deg.C, filtering to obtain filtrate; finally, using neutral proteinase to treat said filtrate, using dialysis bag to make dialysis, making rotary evaporation, utilizing absolute ethyl alcohol to make precipitation and vacuum drying at 40-45deg.C so as to obtain the invented product-light-yellow balsame pear polysaccharide.

Description

technical field [0001] The invention relates to bitter gourd products in the technical field of production technology of agricultural products, in particular to a method for efficiently extracting bitter gourd polysaccharides. Background technique [0002] Momordica charantia (Momordica charantia) is a vine herbaceous plant of the family Cucurbitaceae Momordica charantia, also known as "cool melon", "sweet melon", and "Jin Litchi". It is native to Indonesia and is widely cultivated in Guangdong, Guangxi, Yunnan and other places in my country. Bitter melon is bitter and cold in nature, and has the functions of clearing heat and relieving heat, improving eyesight and detoxification, nourishing kidney and nourishing spleen, etc. It is well known as a melon vegetable that is traditionally used as medicine and food. Modern scientific research has gradually revealed the source of the beneficial effects of bitter melon in theory, and found that various components derived from bitte...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L1/09A23L1/28A23L1/212A23L1/30A23L33/105A23L33/125
Inventor 胡飞陆慧玲
Owner GUANGZHOU JINYANG COSMETICS
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