Anti-senescence product for gonad granulocyte, its preparing method and uses

A granule cell and anti-aging technology, applied in biochemical equipment and methods, anti-toxic agents, microorganisms, etc., can solve the problems of not being able to simulate physiological states well, large differences in physiological states, and single active ingredients, so as to relieve menopausal symptoms, Convenient source and high bioavailability

Inactive Publication Date: 2010-07-07
ANHUI PROVINCIAL HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
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Problems solved by technology

However, these additives all use artificially synthesized estrogen, progesterone or estrogen analogues extracted from plants. big side effects

Method used

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  • Anti-senescence product for gonad granulocyte, its preparing method and uses

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Embodiment Construction

[0029] 1. Obtaining ovarian granulosa cells: 4-6 weeks old female Kunming mice were injected intraperitoneally with 7.5 U of pregnant horse serum (PMSG) per mouse, and they were killed by cervical dislocation after 48 hours of routine feeding. In a petri dish filled with 4°C pre-cooled balanced salt solution (BSS, containing 400IU / ml hyaluronidase, 100U / ml penicillin and streptomycin), puncture the follicles with a 28G injection needle under a stereomicroscope, The granular cells were released into the BSS; the pipette was blown repeatedly several times to disperse them into single cells and filtered through a 200-mesh filter, and the cells were washed twice by centrifugation.

[0030] 2. Acquisition of granulosa cell culture supernatant and cell extract: resuspend granulosa cells in DMEM / F12 medium containing 10% fetal bovine serum by volume, and adjust the cell density to 2×10 6 / ml inoculated culture flasks, cultured at 37°C and 5% CO2 by volume for 6 hours, then changed th...

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Abstract

The invention provides a cellsovary granulosa cell anti-aging product, and the preparation method and the application of the product. The invention applies cellsovary granulosa cells in preparation of anti-aging products. The cellsovary granulosa cell anti-aging product of the invention is characterized in that in vitro cultivated and amplified cultivation supernatant of female mammalian animal celsovary granulose cells and / or the cell extract is contained; and one or more than one pharmaceutically acceptable medical excipient and antioxidant are contained. Via the cellsovary granulosa cells,the invention is capable of proliferating and secreting large amounts of a plurality of bioactive substances. The invention adopts granulose cells with extremely high bioavailability and similar to female physiological characteristics to cultivate supernatant and the cell extract of the supernatant. The invention can be taken orally, absorbed by skin or be prepared into vaginal suppository to achieve the purpose of anti-aging.

Description

technical field [0001] The invention relates to an anti-aging product of ovarian granulosa cells, its preparation method and its application. Background technique [0002] The main function of the ovary is to ovulate and secrete female hormones. It controls several important physiological events in a woman's life: youth development, childbearing, maintenance of good figure and beautiful appearance, among which ovarian granulosa cells play an extremely important role . As the age increases, the ovarian function of women begins to decline around the age of 45-50. Some women experience premature ovarian failure due to environmental, psychological or pathological factors, followed by a series of menopausal symptoms, such as menstrual disorders or menopause, emotional changes, muscle Relaxation, decreased elasticity, dullness, accelerated aging, changes in the body's immune function, increased incidence of cardiovascular diseases, osteoporosis, reproductive system tumors, and ne...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K35/54A61K9/02A61K9/06C12N5/08A61P15/14A61P39/06
Inventor 凌斌冯定庆周颖彭程沈国栋朱园园
Owner ANHUI PROVINCIAL HOSPITAL
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