Novel single-tank hemicontinuous high-strength ferment high optical purity L- lactic acid technique for rhizopus oryzae bacterium ball

A technology of rhizopus oryzae and optical purity, which is applied in the direction of biochemical equipment and methods, fermentation, and microbial-based methods, can solve problems such as industrial production obstruction and immature technology, and achieve N source saving, high fermentation intensity, and reduced The effect of fermentation costs

Inactive Publication Date: 2008-04-02
HEFEI UNIV OF TECH
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Problems solved by technology

[0003] Among numerous lactic acid-producing microorganisms, Rhizopus oryzae (AS 3.819) is an ideal strain for producing L-lactic acid with high optical purity due to its high optical purity, simple nutrient consumption, and easy separation of products. In terms of process control, the technology is immature, and industrial production is hindered

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  • Novel single-tank hemicontinuous high-strength ferment high optical purity L- lactic acid technique for rhizopus oryzae bacterium ball

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Embodiment

[0033] The new process method of rhizopus oryzae sphere single-tank semi-continuous high-intensity fermentation of high optical purity L-lactic acid includes the following process steps:

[0034] (1) Preparation of Rhizopus oryzae spores

[0035] Streak inoculation of Rhizopus oryzae (Rhizopus oryzae / AS 3.819) on a potato dextrose (PDA) plate, set the culture temperature to 32°C on the first day, and set the relative humidity to 70%, which is conducive to the growth of mycelium, and set the culture temperature on the second day It is 34 DEG C, and the relative cultivation humidity is 30%, which is conducive to spore generation. After the black dense spores completely cover the white hyphae, they can be ready to be collected, and mature Rhizopus oryzae spores are obtained.

[0036] (2) prepare high-density and high-activity Rhizopus oryzae spore emulsion suspension:

[0037] Scrape and wash the Rhizopus oryzae spores into sterile water, then inoculate them in a 10L fermenter w...

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Abstract

The invention discloses a novel technique of rhizopus sphere single-pot semi-continuous high-intensity fermentation to produce L-lactic acid with high optical purity, which relates to the procedures including that: firstly, an emulsible suspension of rhizopus pores with high intensity and high actively is prepared; secondly, the rhizopus spheric cells are inoculated in the sterilized condition into a mechanical 500L-mixer for proliferation; thirdly, the first batch of single-pot fermentation is carried out in the 500L pot; fourthly, the single-pot fermentation is repeated in the 500L pot and; fifthly, 15 batches of single-pot semi-continuous high-intensity formation are carried out in the 500L pot. The fermentation technique provides the suspension substrate capable of cultivating rhizopus spheres with regular shape and even size, accomplishes repeated fermentation, economizes the nitrogen source and the other inorganic salt consumed by the bacterium, and decreases the fermentation cost; wherein, the invention has shortened fermentation period, strong fermentation intensity, and suitability for industrialized production; with the product of L-lactic acid with the optical purity above 99.5 percent satisfying the needs on food and medicine, the invention is completely suitable for preparation of poly L-lactic acid with high quality.

Description

technical field [0001] The present invention relates to a new technique for mold fermentation of L-lactic acid. More specifically, the rhizopus oryzae cells have the tendency to aggregate and flocculate to form mycelium balls in a stirred culture system to form fungal spheroids, and then ferment repeatedly through the fungal spheroids, thereby The invention discloses a new process for realizing single-tank semi-continuous high-intensity fermentation of high-optical-purity L-lactic acid with Rhizopus oryzae spheres, which belongs to the field of microorganism and fermentation engineering. Background technique [0002] Lactic acid is one of the three recognized organic acids in the world, and it has a wide range of uses. Lactic acid, lactate and their derivatives are widely used in food, medicine, feed, chemical and other fields. The human body only contains L-lactate dehydrogenase, which can only metabolize L-lactic acid. The use of L-lactic acid in the food industry is abs...

Claims

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Application Information

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IPC IPC(8): C12P7/56C12R1/845
Inventor 姜绍通潘丽军郑志罗水忠李兴江朱羽
Owner HEFEI UNIV OF TECH
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