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Preparation method of pseudo-ginseng protopanoxadiol saponin and its uasage

A technology of protopanaxadiol and Panax notoginseng, which is applied in the direction of pharmaceutical formulations, plant raw materials, and medical preparations containing active ingredients, etc. It can solve problems such as biological activity research, achieve inhibition of angiogenesis, easy quality control, and simple and clear ingredients Effect

Inactive Publication Date: 2008-04-09
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Previously, all studies did not separate the biological activities of panaxadiol and protopanaxatriol saponins in Panax notoginseng

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] The medicinal material of notoginseng is crushed into granular shape (3mm-8mm), extracted twice with 70% ethanol with 10 times the solvent volume, each time for 2 hours. Concentrate the extract to 1g crude drug / ml, apply macroporous resin D101, first wash with water for 3-5 column volumes (BV), then wash with 0.05-0.1% ammonia water for 4-5BV, then wash with water until pH = 7, and then use Wash 3BV with 40% ethanol, and finally elute 3-5BV with 70% ethanol, collect the 70% ethanol eluate, concentrate and dry. Dissolve the sample in water, put it on the reversed-phase packing column ODS C18, first elute 3-4 BV with 20-25% ethanol, then elute 3-5 BV with 40-50% ethanol, collect the eluate, recover the solvent, and dry , after dissolving in water, apply macroporous resin D1 01, elute 3BV with 30% and 70% ethanol respectively, collect 70% ethanol eluate and dry it.

Embodiment 2

[0044] The medicinal material of notoginseng is crushed into granular shape (3mm-8mm), extracted twice with 70% ethanol with 10 times the solvent volume, each time for 2 hours. Concentrate the extract to 1g crude drug / ml, apply macroporous resin D101, first wash with water for 3-5 column volumes (BV), then wash with 0.05-0.1% ammonia water for 4-5BV, then wash with water until pH = 7, and then use Wash 3BV with 40% ethanol, and finally elute 3-5BV with 70% ethanol, collect the 70% ethanol eluate, concentrate and dry. Dissolve the sample in water, put it on the reversed-phase packing column ODS C8, first elute 3-4 BV with 15-20% ethanol, then elute 3-5 BV with 20-40% ethanol, collect the eluate, recover the solvent, and dry , after dissolving in water, apply macroporous resin D101, elute 3BV with 30% and 70% ethanol respectively, collect 70% ethanol eluate and dry it.

Embodiment 3

[0046] The medicinal material of notoginseng is crushed into granular shape (3mm-8mm), extracted twice with 70% ethanol with 10 times the solvent volume, each time for 2 hours. Concentrate the extract to 1g crude drug / ml, apply macroporous resin D201, first wash 3-5 column volumes (BV) with water, then wash 3BV with 30% ethanol, and finally elute 3-5BV with 70% ethanol, collect 70% The ethanol eluate was concentrated and dried. Dissolve the sample in water, put it on the reversed-phase packing column ODS C18, first elute 3-4 BV with 20-25% ethanol, then elute 3-5 BV with 40-50% ethanol, collect the eluate, recover the solvent, and dry , after dissolving in water, apply macroporous resin D101, elute 3BV with 30% and 70% ethanol respectively, collect 70% ethanol eluate and dry it.

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PUM

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Abstract

The invention provides a preparation method for extractive originating from an araliaceae plant, notoginseng. The extractive of the category mainly contains extractive of dammarane type, tetracyclic triterpene category, protopanaxadiol type and saponin category, wherein the content of ginsenoside Rb1 and Rd is not lower than 60 percent, and the content of ginsenoside Re, Rg1, and notoginseng saponin R1 is not higher than 5 percent. The notoginseng is extracted by using alcohol containing water as the solvent, chromatography to the extracting solution through a chromatographic column is performed to obtain elutriant, reversed phase and liquid phase gradient elution is performed, the elutriant is collected, and then the target extractive is obtained through concentration. The extraction process of the invention can effectively remove impurities of sugar, protein, amino acid, and the saponin of the protopanaxadiol type of the notoginseng, etc., and improve the content of effective components. The effective components are definite, the process is simple, the quality is controllable, and the invention is suitable for industrialized production, thereby being applied for preparing medicines treating malignant solid tumors.

Description

technical field [0001] The invention belongs to a traditional Chinese medicine extract, and relates to a diol type dammarane tetracyclic triterpene saponin extract extracted from the traditional Chinese medicine Panax notoginseng. The extract has the function of inhibiting angiogenesis and can be used for the prevention and treatment of malignant solid tumors. technical background [0002] When the size of the tumor exceeds 0.2 mm, it begins to establish its own blood supply system, that is, new angiogenesis (angiogenesis). Angiogenesis is the growth of new capillary blood vessels, and this process mainly depends on the migration, proliferation and lumen formation of capillary endothelial cells. Under normal physiological conditions, angiogenesis is strictly controlled by the balance between endogenous angiogenic factors and anti-angiogenic factors; while the "angiogenesis initiation" of tumors is the precursor of oncogene activation of tumor expression of angiogenic factors...

Claims

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Application Information

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IPC IPC(8): A61K36/258A61P35/00
Inventor 刘雳贺庆程翼宇水文波葛志伟窦静霍杨
Owner ZHEJIANG UNIV
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