Novel use for Candida tropicalis

A technology of Candida tropicalis, yeast extract, applied in the direction of fermentation, microorganism-based methods, microorganisms, etc., to achieve the effect of reducing costs

Inactive Publication Date: 2008-04-23
CAPITAL NORMAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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  • Novel use for Candida tropicalis
  • Novel use for Candida tropicalis
  • Novel use for Candida tropicalis

Examples

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Example Embodiment

[0045] Example 1 Isolation and identification of Y1

[0046]Y1 separation method: Collect soil samples from a depth of 1-5 cm below the soil surface near a furfural plant, activate the collected soil samples and add them to YPD medium for enrichment culture, and then take 10 mL of enriched bacteria The liquid was respectively connected to the medium containing 90mL hydrolyzate (yeast extract 3g / L, KH 2 PO 4 3g / L, malt extract 3g / L, (NH 4 ) 2 SO 4 1g / L, MgSO 4 .7H 2 01g / L, tryptone 3g / L, the rest are lignocellulose hydrolyzate stock solution, adjust pH to 5.0-6.0) in a 250mL conical flask, cultivate at 80rpm and 30℃ for 48h, and then take 10mL of this culture solution and put it into 90mL In the same fresh medium, continue to culture at 80rpm and 30°C for 48h, repeat this, after 4-5 transfers, take 10uL of the culture solution after a certain gradient dilution and spread it on the same medium solid plate, After culturing at 30°C for 48 hours, a single colony of typical ...

Example Embodiment

[0064] Example 2 Ethanol fermentation of Y1

[0065] Y1 strain was inoculated into the yeast extract containing 3g / L, KH 2 PO 4 3g / L, malt extract 3g / L, (NH 4 ) 2 SO 4 1g / L, MgSO 4 .7H 2 0.5g / L, tryptone 3g / L, hydrolyzate stock solution, in the medium of pH5.0-5.5, the glucose was used up within 24h, and in the fermentation process, the xylose also gradually decreased, after 72 hours, the xylose was Sugar is completely consumed. The highest yield of ethanol reached 96.1% of the theoretical value (theoretical yield was 0.51 g / g). Fermentation conditions were 30°C, 80 rpm.

Example Embodiment

[0066] Example 3 Xylitol fermentation of Y1

[0067] The conditions of the xylitol fermentation of Y1: ammonium sulfate 10g / L, potassium dihydrogen phosphate 3g / L, magnesium sulfate 1g / L, yeast extract 3g / L, the rest are lignocellulose hydrolyzate stock solution, adjust pH to 5.0, ferment Conditions: 30℃, 150rpm, fermentation for 72 hours, the conversion rate of xylitol is 0.65g.g -1 .

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Abstract

The present invention provides the application of Candida tropicalis in fermenting lignocellulose hydrolysate. Candida tropicalis and its domesticated strain is applied in fermenting lignocellulose hydrolysate to degrade the fermentation inhibitor fast, to metabolize glucose for producing ethanol, to convert xylose into xylitol and to lower the cost of producing ethanol with lignocellulose. The present invention lays foundation of large scale industrial production, and provides also one Candida tropicalis strain Y1 with high fermentation efficiency.

Description

technical field [0001] The invention relates to a new application of Candida tropicalis, in particular to the application in the fermentation of lignocellulose hydrolyzate. Background technique [0002] Producing ethanol from non-food material-lignocellulosic raw materials is one of the important ways to ensure national energy security, protect the environment, and make my country's economic and social sustainable development. The composition of the lignocellulose hydrolyzate: glucose+xylose mixed sugar concentration 6%, acetic acid 10g / L, furfural 2g / L, hydroxymethylfurfural 1g / L, and contains phenolic compounds and aromatic hydrocarbon compounds. Efficient and rapid hydrolysis of lignocellulose and efficient ethanol fermentation of hydrolyzed products are two technical bottlenecks in the industrial production of fuel ethanol at home and abroad. In addition to mixed sugars, lignocellulose hydrolyzate also contains fermentation inhibitors such as furfural, hydroxymethylfurf...

Claims

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Application Information

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IPC IPC(8): C12N1/16C12P7/10C12P7/18C12R1/74
CPCY02E50/16Y02E50/10
Inventor 杨秀山
Owner CAPITAL NORMAL UNIVERSITY
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