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Composite preparation made from microorganism fermented liquid

A technology for microbial fermentation liquid and preparation, applied in the field of microbiology, can solve the problems of no preventive activity against plant diseases, no control of bacterial infection, plant damage, etc.

Inactive Publication Date: 2008-04-30
黄永
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

S.D. Savag et al. applied fatty acids to the diseased parts of plants to directly kill fungi and bacteria and achieved good results (CN1069391A). Their research emphasized the therapeutic effect of fatty acids on plant diseases by killing fungi and bacteria, but made a statement Fatty acids are inactive at very low concentrations and harmful to plants at high concentrations, and fatty acids and their salts or derivatives have no preventive activity against plant diseases
In the experimental results given by them, fatty acids and its salts or derivatives are directly applied to the diseased parts of plants to play a therapeutic role, and the minimum effective concentration of fatty acids and its salts or derivatives applied to plants is 0.125% a.i. (1250 mg / L), fatty acids or their salts or derivatives used at concentrations as low as 0.063% have no effect in controlling the infestation

Method used

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  • Composite preparation made from microorganism fermented liquid

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0018] 1) Add 20 grams of glucose and malt extract powder, 5 grams of yeast extract powder, 5 grams of calcium trehalose ammonia, 15 grams of agar, and add water to 1 liter. After being sterilized and cooled, pour it into a petri dish to make a culture medium (GMYAA). Streak culture on the culture medium with Pseudomonas glathei TP06, a related strain selected from the genus Pseudomonas, was cultured for 48 hours to obtain strains.

[0019] 2) Transfer the strain from the culture medium to the GMYAB-containing culture medium (20 grams of glucose and malt extract powder each, add 5 grams of yeast extract powder, 5 grams of calcium trehalose ammonia, add water to 1 liter), at a temperature of 30 ° C and a rotation speed of 125 rpm Incubate on a shaker for 72 hours under optimal conditions.

[0020] 3) Transfer the cultured strains to a fermenter with a 10% inoculum amount, and use the same culture solution as that in the seed tank for aerated fermentation at 30° C. for 72 hours...

example 2

[0022] Example 2. The content of fatty acid in the multi-effect preparation was determined by gas chromatography-mass spectrometry according to the following conditions.

[0023] 1. Instrument Agilent Technologies GC / MS 5975i

[0024] 2. Chromatographic column: HP-5ms (30m×250μm×0.25μm) 5%PhenyMethylSiloxane

[0025] 3. Chromatographic column temperature program: keep at 100°C for 1min, then program temperature up to 130°C at 30°C / min, then program temperature up to 250°C at 5°C / min, then program temperature up to 300°C at 10°C / min, keep for 5min ;

[0026] 4. Carrier gas: helium, purity ≥ 99.999%, flow rate 1.2mL / min;

[0027] 5. Inlet temperature: 290°C;

[0028] 6. Injection volume: 2μL;

[0029] 7. Sampling method: non-split sampling;

[0030] 8. Electron bombardment source: 70eV;

[0031] 9. Quadrupole temperature: 150°C;

[0032] 10. Ion source temperature: 230°C;

[0033] 11. GC-MS interface temperature: 280°C;

[0034] Full spectrum scan; mass scan range m / z: ...

example 3

[0038] Example 3. The effect of multi-effect preparation on promoting plant growth and increasing yield

[0039] Take 100 ml of the multi-effect preparation, dilute it with water 100 times, and evenly spray it on the newly transplanted eggplant leaves in the field, spray once every 15 days, do not use any chemical fungicides and topdressing, and spray chemical pesticides and topdressing on adjacent plots conventional management as a comparison. The results are shown in Table 2. The multi-effect preparation significantly promotes the growth of crops, and obtains a yield increasing effect of up to 42%.

[0040] Table 2 Comparison of effects of multi-effect preparations on promoting growth and increasing yield of eggplant

[0041]

[0042] Note 1: Different letters after the numbers in the same column indicate significant differences between treatment and control.

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Abstract

A multiple-effect medicament compounded by microbe fermentation liquor has a multiple-effect medicament formulation compounded by microbe fermentation liquor and inorganic acid or organic acid, and inorganic salt or organic salt. The multiple-effect medicament of the compounded medicament comprises very obvious characteristic component of aliphatic acid. The multiple-effect medicament realizes multiple effects of promoting plants growing, increasing plants volume of output, preventing and curing plants diseases, and is a multiple-effect medicament which can promote plants to grow, increase plants volume of output, has preventing and curing effects for plant diseases.

Description

1. Technical field [0001] The invention relates to a new application of microbial fermented liquid in plant protection and plant growth promotion, belongs to the technical fields of microbiology, chemistry and agriculture, and involves microbiology, chemistry, agronomy and biochemistry and the like. 2. Technical background [0002] With the development of the chemical industry and the expansion of the use of pesticides and fertilizers, the quantity and variety of chemical pesticides are increasing. Now the total output of chemical pesticides in the world (in terms of active ingredients) exceeds 3 million tons. Due to the long-term and large-scale application of chemical pesticides and chemical fertilizers, serious environmental pollution and increasing resistance of diseases and insect pests have been brought about. Many countries have invested a lot of money in the research and development of new formulations that can replace or reduce chemical pesticides and fertilizers, s...

Claims

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Application Information

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IPC IPC(8): A01N63/02A01P21/00A01N37/00A01N59/00
Inventor 黄永
Owner 黄永
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