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Adenosine deaminase diagnosis reagent kit and adenosine deaminase activity and concentration determination method

An adenosine deaminase and diagnostic kit technology, which is applied in the field of determination of adenosine deaminase activity concentration and adenosine deaminase diagnostic kits, and can solve problems such as difficult popularization, application and determination.

Inactive Publication Date: 2008-04-30
SUZHOU ANJ BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this method cannot be determined by the visible light analyzer in general hospitals, but requires the use of a special ultraviolet light analyzer, so it is difficult to practically popularize and apply

Method used

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  • Adenosine deaminase diagnosis reagent kit and adenosine deaminase activity and concentration determination method

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] The adenosine deaminase diagnosis / measurement reagent of this embodiment is a single reagent, including:

[0039] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0040] Stabilizer 50mmol / L

[0041] Reduced coenzyme 0.25mmol / L

[0042] Alanine dehydrogenase 10000U / L

[0043] Glycine oxidase 12000U / L

[0044] Adenosine 6mmol / L

[0045] Pyruvate 12mmol / L

[0046] After the reagents are all dissolved and prepared, they are divided into bottles and freeze-dried to make dry powder reagents; before use, add purified water and reconstitute for use.

[0047] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance 1.8±0.7, test main wavelength 340nm, test secondary wavelength 405nm, the volume ratio of the tested adenosine deaminase sample to the reagent is 1 / 25, the reaction direction is negative reaction (falling reaction), the delay time is about 1 minute, the detection time is about 2 minutes, and th...

Embodiment 2

[0050] The adenosine deaminase diagnosis / measurement reagent of this embodiment is a double reagent, comprising:

[0051] Reagent 1

[0052] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0053] Stabilizer 50mmol / L

[0054] Reduced coenzyme 0.25mmol / L

[0055] Adenosine 8mmol / L

[0056] Pyruvate 16mmol / L

[0057] Reagent 2

[0058] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0059] Stabilizer 50mmol / L

[0060] Alanine dehydrogenase 12000U / L

[0061] Glycine oxidase 16000U / L

[0062] After the reagents are all dissolved and prepared, they are divided into bottles to make liquid double reagents, which can be used directly.

[0063]Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance 1.8±0.7, test main wavelength 340nm, test secondary wavelength 405nm, tested adenosine deaminase sample and reagent 1, reagent 2 The volume ratio is 2 / 20 / 5, the reaction direction is negative...

Embodiment 3

[0066] The adenosine deaminase diagnosis / measurement reagents of this embodiment are three reagents, including:

[0067] Reagent 1

[0068] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0069] Stabilizer 50mmol / L

[0070] Reduced coenzyme 0.25mmol / L

[0071] Reagent 2

[0072] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0073] Stabilizer 50mmol / L

[0074] Adenosine 4mmol / L

[0075] Pyruvate 20mmol / L

[0076] Reagent 3

[0077] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0078] Stabilizer 50mmol / L

[0079] Alanine dehydrogenase 8000U / L

[0080] Glycine oxidase 12000U / L

[0081] After all the reagents are dissolved and prepared, they are divided into bottles to make liquid three reagents, which can be used directly.

[0082] When measuring the activity concentration of adenosine deaminase, set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance 1...

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Abstract

The invention relates to a adenylate deaminase diagnostic reagent box utilizing the enzyme cycle amplification, the enzyme-colorimetric and the enzyme-linked techniques, meanwhile, the invention also relates to a method for detecting the activity consistence of the adenylate deaminase, and the compositions and the component of reagent, and belongs to the technical field of medical examination and determination. The reagent box provided by the invention contains buffer solution, reduced coenzyme, adenosine, pyruvic acid, alanine dehydrogenase, glycine oxidase and stabilizer. The sample is mixed with the reagent at certain volume ratio to impel the series of enzymatic reaction between the sample and the reagent, and the reactant is then put under an analyzer of visible light / ultraviolet ray to detect the descending speed of absorbency at the 340nm of the main wavelength, thus, the activity consistence of the adenylate deaminase can be calculated. The invention can completely obtain the needed test result with the help of the analyzer of visible light / ultraviolet ray.

Description

technical field [0001] The invention relates to a diagnostic kit for adenosine deaminase, and at the same time, the invention also relates to a method for measuring the activity concentration of adenosine deaminase, which belongs to the technical field of medical examination and measurement. Background technique [0002] Medical research shows that adenosine deaminase is a nucleic acid metabolizing enzyme that has an important relationship with the body's cellular immune activity. Therefore, the determination of adenosine deaminase activity is used as an important diagnostic marker for the differential diagnosis of benign and malignant hydrothorax, cerebrospinal fluid, severe combined immunodeficiency disease (SCID), acute and chronic hepatitis, liver cirrhosis, liver cancer and other diseases. [0003] The methods for determining the activity of adenosine deaminase mainly include radionuclide method, physical method and biochemical method. As far as the applicant knows, th...

Claims

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Application Information

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IPC IPC(8): G01N21/78G01N21/25C12Q1/32C12Q1/68C12Q1/00
Inventor 王尔中
Owner SUZHOU ANJ BIOTECHNOLOGY CO LTD
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