High activity, high-purity and high temperature resistant Alpha-amylase production process

Abstract

The invention provides a production process that bacteria strain (12 hours)to a first-class seed tank (28 hours)to big tank (about 100 hours, 12000u / ml draw off enzyme activity)to post treatment of flocculation (70 degree)-pressure filtration-cycle initial filtration-ultra-filtration to fine filtration (10 hours) to final product treatment (elevated temperature 80 degree) to precipitation (48 hours) to alpha-amylase production process with high activity, high purity and high temperature resistance. The XF-19 strain is selected; a secondary fermentation process, the addition of feeding material into the basic material, continuous feeding of ammonia and the application of cooked soybean meal as nitrogen source are applied during the production process; the cycle initial filtration and fine filtration are added in the post treatment process, which solves the shortcomings of old production process such as weak enzyme production ability per unit, decreasing pH value during auto-metabolism of the enzyme seed, little enzyme activity, poor temperature resistance, easy precipitation and high total bacteria count etc. The invention can reduce the production cost, great economic benefits and big popularization and application value.

Description

technical field [0001] The invention relates to a production process of a bioengineering enzyme preparation, in particular to a production process of a high-activity, high-purity, high-temperature-resistant α-amylase. Background technique [0002] High-temperature-resistant α-amylase is refined by Bacillus licheniformis through submerged liquid culture and extraction process. It has excellent heat resistance and can randomly hydrolyze α-amylase in starch, soluble dextrin and oligosaccharides at higher temperatures. -1.4 glucosidic bond, the viscosity of colloidal starch is rapidly reduced, hydrolyzed to generate dextrin and a small amount of glucose and maltose. It is widely used in alcohol, beer, starch sugar, liquor, pharmaceutical and textile industries, and is currently the most widely used enzyme in industry. At present, the production process of domestic high-temperature-resistant α-amylase is: strain (24h) → first-class seed tank (20h) → large tank (about 80h, the en...

AI Technical Summary

Problems solved by technology

Its shortcomings are: ① Reasonable carbon-nitrogen ratio, dissolved oxygen and osmotic pressure cannot be guaranteed during the fermentation process, so it is difficult to meet the optimal metabolism and enzyme production conditions of the enzyme species, and the enzyme production capacity of the enzyme species cannot reach the best effect

②The product quality is extremely unstable, the color varies from thick to light, the clarity varies from thick to clear, easy to produce sediment, the pH value is unstable, and the total number of bacteria exceeds the standard