Hippophae rhamnoides nutrition mixture with delaying senility function and preparation technique thereof
A nutrient mixture and anti-aging technology, which is applied in the direction of medical preparations containing active ingredients, anti-toxins, pill delivery, etc., and can solve problems such as single function, poor absorbability, and limited medicinal effect
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Embodiment 1
[0028] 1) Extraction of seabuckthorn polypeptide: First, weigh 1000g of seabuckthorn fruit, put it into a masher, add 500ml of water, and mash it into a seabuckthorn slurry. Then put the seabuckthorn slurry into a 2000ml beaker, put the beaker into a water bath, heat up to 50°C, adjust its pH to 7.5 with sodium hydroxide, and then add 1% of the weight of the seabuckthorn fruit. Protease is used for enzymatic hydrolysis. Neutral protease can be selected from Novozymes, Denmark. The enzyme activity unit is 200,000 U / g. It has high specificity, stable performance, and stable enzymatic hydrolysis products. During the enzymolysis process, the temperature was maintained at 50° C., the pH value was 7.5, and the duration was 2 hours to obtain seabuckthorn enzymolysis slurry. Finally, a centrifuge was used to separate the seabuckthorn enzymolysis slurry from solid to liquid at a rate of 3000r / min, and the treatment time was 20 minutes. After solid-liquid separation, the supernatant wa...
Embodiment 2
[0032]1) Extraction of seabuckthorn polypeptide: First, weigh 1000g of seabuckthorn fruit, put it into a masher, add 400ml of water, and mash it into a seabuckthorn slurry. Then put the seabuckthorn slurry into a 2000ml beaker, put the beaker into a water bath, heat up to 45°C, adjust its pH to 8.0 with sodium hydroxide, and then add 2% of the weight of the seabuckthorn fruit. The enzymolysis was carried out by protease, and the temperature was maintained at 45° C., the pH value was 8.0, and the duration was 4 hours during the enzymolysis process to obtain seabuckthorn enzymolysis slurry. Finally, a centrifuge was used to separate the seabuckthorn enzymolysis slurry from solid to liquid at a rate of 3500r / min, and the processing time was 15 minutes. After solid-liquid separation, the supernatant was taken, conventionally concentrated and vacuum-dried to obtain 596 g of seabuckthorn polypeptide, with a yield of about 60%.
[0033] 2) Extraction of seabuckthorn flavonoids: Firs...
Embodiment 3
[0036] 1) Extraction of seabuckthorn polypeptide: First, weigh 1000g of seabuckthorn fruit, put it into a masher, add 600ml of water, and mash it into a seabuckthorn slurry. Then put the seabuckthorn slurry into a 2000ml beaker, put the beaker in a water bath, heat up to 60°C, adjust its pH to 8.0 with sodium hydroxide, and then add 3% of the weight of the seabuckthorn fruit. Enzymolysis was carried out with protease. During the enzymolysis process, the temperature was maintained at 60° C., the pH value was 8.0, and the duration was 6 hours to obtain seabuckthorn enzymolysis slurry. Finally, a centrifuge was used to separate the seabuckthorn enzymolysis slurry from solid to liquid at a rate of 4000r / min, and the processing time was 20min. After solid-liquid separation, the supernatant was taken, conventionally concentrated and vacuum-dried to obtain 695 g of seabuckthorn polypeptide, with a yield of about 70%.
[0037] 2) Extraction of seabuckthorn flavonoids: First, weigh 50...
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