Method for plants radioinduction

A plant and plant seed technology, applied in the fields of botany equipment and methods, plant genetic improvement, application, etc., can solve the problems of high seed mortality and low mutation rate, and achieve low seed mortality, high mutation rate, and processing time. short effect

Inactive Publication Date: 2008-06-11
PEKING UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In order to solve the problem of high seed mortality and low mutation rate after traditi...

Method used

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  • Method for plants radioinduction
  • Method for plants radioinduction
  • Method for plants radioinduction

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1, H + Ion Beam Irradiation of Arabidopsis Seeds Produces Mutants

[0026] Select 10,000 Arabidopsis seeds with full grains, no damage, and no pollution

[0027] The selected seeds are naturally adsorbed on wet filter paper and placed on the outer target disk of the accelerator. The accelerator beam is extracted from the vacuum chamber through a 6.5 μm thick titanium membrane.

[0028] Choose H with an energy of 2.0 MeV in vacuum + The ion beam is irradiated, and the energy drops to 1.1MeV when the ions pass through 2mm of air to reach the seed epidermis, and the penetration depth is 30 μm, while the stem apical meristem is 80 μm away from the epidermis. The incident ion fluence range is 1×10 10 ions / cm 2 to 1×10 13 ions / cm 2 .

[0029] The irradiated seeds were planted in the soil, and the growth was observed, and the unirradiated Arabidopsis seeds were used as the control. The results showed that 8832 seeds germinated 14 days after sowing the irradiat...

Embodiment 2

[0030] Example 2, He 2+ Mutants Produced by Ion Beam Irradiation of Tobacco Seeds

[0031] Choose complete, full-bodied tobacco seeds about 1000 grains. Place on wet filter paper for three days at 4°C. Saturate the seeds with water.

[0032] A gap with a width of about one-third of the tobacco seeds is etched on the silicon wafer by a fine processing method, and the seeds are fixed and arranged on the other side of the gap, so that the gap is aligned with the radicle part of the seed, and the stem end meristem It is in the shielding part of the silicon wafer, and then chooses He with an energy of 10.0MeV in vacuum 2+ The ion beam is irradiated from the other side of the silicon wafer. When the ions pass through the 6.5μm Ti film and the air layer of about 3mm and reach the seed skin, the energy drops to 8.5MeV, and the incident ion fluence ranges from 1×10 10 ions / cm 2 to 1×10 13 ions / cm 2 .

[0033] The irradiated seeds were cultured at 23°C, and the unirradiated toba...

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Abstract

The invention discloses a method for plant radiating mutagenesis. Seeds are sewed and screened to gain mutants after wave rays are utilized to radiate peripheral cells of the stem apex meristem of seeds of plants which are damaged. The invention has the advantages that firstly, the penetration of a shallow layer of wave rays has relatively little damage to fetus of seeds, thereby death rate of seeds is very low, which is equal to improving the mutagenesis base, secondly, wave ray functions of different varieties and different energy can generate small molecules of different signals which are conducted to the stem apex meristem area and arouse bond breaking of corresponded DNA molecules, and mutation varieties which are numerously generated have the characteristic of directional mutation, thirdly, the process time is short and the mutation efficiency is high, fourthly, toxic substances are not involved in the invention, and the invention has no toxicity and harm, is safe and protects the environment, fifthly, a high-energy accelerator is not needed, which greatly reduces cost.

Description

technical field [0001] The invention relates to a method for plant radiation mutagenesis. Background technique [0002] Mutagenesis is a basic method for the study of plant gene regulation and the cultivation of new varieties. Currently commonly used methods can use physical factors, chemical factors, etc. to achieve. The physical factor is mainly to directly penetrate the plant seeds with high-energy rays and directly irradiate and damage the genetic material in the stem meristem cells. However, the disadvantage of this method is that the lethal rate is too high, the mutation frequency is too low, and the stability of the mutant is not good. Chemical factors are mainly soaked in reagents to make chemical molecules interact with intracellular molecules. The disadvantage is that it causes greater physiological damage to plants. Contents of the invention [0003] In order to solve the problems of high seed mortality and low mutation rate after traditional radiation treatm...

Claims

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Application Information

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IPC IPC(8): A01H1/06
Inventor 王宇钢梅韬覃怀莉薛建明顾红雅
Owner PEKING UNIV
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