Method for directly analyzing chemical composition of plant tissue slice

A plant tissue and chemical composition technology, applied in the analysis of materials, material analysis by electromagnetic means, scientific instruments, etc., can solve the problems of cumbersome identification process, large demand for samples, difficult identification and analysis, etc., to achieve high sensitivity and reduce analysis. cost, effect of avoiding extraction steps

Inactive Publication Date: 2008-06-11
蔡宗苇
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  • Application Information

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Problems solved by technology

Most samples require large quantities, low sensitivity and require a tedious identification pr...

Method used

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  • Method for directly analyzing chemical composition of plant tissue slice
  • Method for directly analyzing chemical composition of plant tissue slice
  • Method for directly analyzing chemical composition of plant tissue slice

Examples

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Effect test

Embodiment 1

[0022] Select 1 Chuanwu and soak it in water for 10 minutes. Cut the plants into 10-20 μm thick slices with a microtome. Put it on the target of matrix-assisted laser desorption ionization-time-of-flight mass spectrometer (MALDI-TOF-MS), such as figure 1 shown. Freshly prepare the matrix solution before the experiment: acetonitrile / 0.1% trifluoroacetic acid (50:50) and α-cyano-4-hydroxycinnamic acid (CHCA, α-cyano-4-hydroxycinnamic acid) to make a saturated solution (~30mg / ml) spare. Add the matrix solution to the plant tissue slices with a pipette at a volume of about 30 μl / cm 2 . After the matrix solution is spotted on the tissue section, the target is placed in a vacuum desiccator and dried for measurement. Use matrix-assisted laser desorption ionization-time-of-flight mass spectrometer (MALDI-TOF-MS) for direct measurement, try to use smaller laser energy during the measurement process, such as 9-13μJ, select 10 points clockwise on the slice, each Each point is coll...

Embodiment 2

[0024] Take 1 Aconitum aconitum and soak it in water for 10 minutes. Cut the plants into 10-20 μm thick slices with a microtome. Put it on the target of matrix-assisted laser desorption ionization-time-of-flight mass spectrometer (MALDI-TOF-MS), such as figure 1 shown. Freshly prepare the matrix solution before the experiment: acetonitrile / 0.1% trifluoroacetic acid (50:50) and α-cyano-4-hydroxycinnamic acid (CHCA, α-cyano-4-hydroxycinnamic acid) to make a saturated solution (~30mg / ml) spare. Use a pipette to add the matrix solution to the plant tissue section with an amount of about 30 μl / cm 2 . After the matrix solution is spotted on the tissue section, the target is placed in a vacuum desiccator and dried for measurement. Matrix-assisted laser desorption time-of-flight mass spectrometry (MALDI-TOF-MS) was used for direct measurement, and the smaller laser energy was used as much as possible during the measurement process. 10 points were selected clockwise on the slice,...

Embodiment 3

[0026] Select 1 Nuxychnium seed and soak it in water for 10 minutes. Cut the plants into 10-20 μm thick slices with a microtome. Put it on the target of matrix-assisted laser desorption ionization-time-of-flight mass spectrometer (MALDI-TOF-MS), such as figure 1shown. Freshly prepare the matrix solution before the experiment: acetonitrile / 0.1% trifluoroacetic acid (50:50) and α-cyano-4-hydroxycinnamic acid (CHCA, α-cyano-4-hydroxycinnamic acid) to make a saturated solution (~30mg / ml) spare. Use a pipette to add the matrix solution to the plant tissue section with an amount of about 30 μl / cm 2 . After the matrix solution is spotted on the tissue section, the target is placed in a vacuum desiccator and dried for measurement. Matrix-assisted laser desorption ionization-time-of-flight mass spectrometer (MALDI-TOF-MS) was used for direct measurement, and as little laser energy as possible was used in the measurement process. Select 10 points clockwise on the slice, collect 3...

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Abstract

The invention is a method of directly analyzing the chemical composition of plant tissue slice, which relates to the chemical composition analyzing technology and comprises the following procedures: S1. Cutting the plant for testing into the slice of 10-20 Mu m thickness, and taking a piece of slice to lay on the target of stroma auxiliary laser analyzing flying time mass spectrograph; S2. Preparing stroma solution; S3. Using liquid displacing machine to add the stroma solution on the plant tissue slice; S4. Laying the target and the tissue slice dotted with stroma solution in vacuum dryer to dry; S5. Directly putting the target and the plant tissue slice for testing after drying into the stroma auxiliary laser analyzing flying time mass spectrograph to conduct analyzing, and finally achieving the information of the chemical composition contained by the plant slice. The analyzing method of the invention is convenient, quick and high-efficient, which doesn't need sample chemical processing, thus avoiding the defects that the traditional sample processing and analyzing method wastes long time, and the microcomposition is difficult to analyze and is possible to damage the chemical composition.

Description

technical field [0001] The invention relates to the technical field of chemical component analysis, and belongs to a method for directly analyzing chemical components of plant tissue slices. Background technique [0002] For the study of the chemical constituents of traditional Chinese medicine, the traditional method is to use solvent distribution and column chromatography to separate them into pure single compounds one by one, and then carry out structure identification. The separation of active ingredients mainly depends on chromatography, such as gas chromatography, high performance liquid chromatography, etc. (Chen Yaozu, Chen Shaoyuan. An introduction to chemical methods in the modernization of traditional Chinese medicine, Science and Technology Press, Beijing, 2003, 5-6). Using this method, many traditional Chinese medicine components can be elucidated. This technology has achieved fruitful results in the research of chemical components of traditional Chinese medic...

Claims

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Application Information

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IPC IPC(8): G01N27/64G01N30/00
Inventor 蔡宗苇吴巍赵中振梁之桃
Owner 蔡宗苇
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