Method and isolated device for micro flux liquid chromatogram on-line large volume sample injection

A technology of large-volume sampling and liquid chromatography, applied in the field of online large-volume sampling of micro-flow liquid chromatography, to achieve the effect of easy realization, easy automation and stable method

Active Publication Date: 2011-08-10
中科榆林能源技术运营有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It overcomes the gradient delay effect caused by large-volume injection in the micro-flow system, and is especially suitable for gradient separation

Method used

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  • Method and isolated device for micro flux liquid chromatogram on-line large volume sample injection
  • Method and isolated device for micro flux liquid chromatogram on-line large volume sample injection
  • Method and isolated device for micro flux liquid chromatogram on-line large volume sample injection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Such as figure 1 As shown, a method and device for micro-flow HPLC online large-volume sampling, consisting of a pump 1, a first splitter 2, a sampling device 3, a second splitter 4, a switching device 5, a pressure control device 6, and a detector 7 and separation column 8 to form.

[0026] The pump 1 is connected to the first splitter 2, and then connected to the second splitter 4 through the sampling device 3, and the two splitters are connected to the switching device 5 at the same time, and finally the flow path passes through a pressure control device 6 into waste liquid. The separation column 8 is directly connected with the second splitter 4, which can reduce the dead volume before the column; the detector 7 is an on-column detector.

[0027] During sample injection, the second splitter 4 is closed by switching the device 5, the flow is split by the first splitter 2, and the sample is introduced into the separation column by the sampling device 3 at a low flow ...

Embodiment 2

[0030] Such as figure 2 As shown, a method and device for micro-flow HPLC online large-volume sampling, consisting of a pump 1, a first splitter 2, a sampling device 3, a second splitter 4, a switching device 5, two pressure control devices 6, The detector 7 and the separation column 8 are composed.

[0031] The pump 1 is connected to the first splitter 2, and then connected to the second splitter 4 through the sampling device 3; the first splitter 2 and the second splitter 4 are respectively connected to a pressure controller 6, and finally connected to the switching device 5 Above; the separation column 8 is directly connected to the second splitter 4, which can reduce the dead volume before the column; the detector 7 is an on-column detector.

[0032] During sample injection, the second splitter 4 is closed by the switching device 5 , and the flow is split by the first splitter 2 . The sample is introduced into the separation column by the sampling device 3 at a low flow...

Embodiment 3

[0035] Such as image 3 As shown, a method and device for micro-flow HPLC online large-volume sampling, consisting of a pump 1, a first splitter 2, a sampling device 3, a second splitter 4, two switching devices 5, and two pressure control devices 6. The detector 7 and the separation column 8 are composed.

[0036] The pump 1 is connected with the first flow divider 2, and then connected with the second flow divider 4 through the sampling device 3; the first flow divider 2 and the second flow divider 4 are respectively connected with a pressure controller 6 and a switching device 5, and the flow The road flows into the waste liquid through them into two passages; the separation column 8 is directly connected with the second splitter 4, which can reduce the dead volume before the column; the detector 7 is a UV detector on the column.

[0037] During sample injection, the second splitter 4 is closed by the switching device 5 connected to the second splitter 4 . The flow is spl...

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Abstract

The invention relates to a sampling method of a micro-separation system, in particular to a micro-flow liquid phase chromatogram online large-volume sampling method and a special device, wherein, a multi-flow path shunt switching device is arranged inside the flow path of a micro-flow liquid phase chromatogram separation system to ensure that mobile phase can be split from different flow paths during system sampling and separation; moreover, large-volume sampling of a chromatogram system is realized through retaining the sample on stationary phase; namely, the mobile phase, which is split by a splitter, enters into a sampling device and then enters into a separation column via another splitter; moreover, the sample is led in the separation column from the sampling device at a low flow rate. The invention has the advantages that: the method can realize the sampling of large-volume sample inside a micro-flow separation system; moreover, the method also overcomes the gradient delay effect caused by large-volume sampling inside a micro-flow system, and is particularly suitable for gradient separation. In addition, the system not only has the characteristics of small dead volume, smallpre-column effect and outstanding separation repeatability, but also has a simple structure and convenient operation.

Description

technical field [0001] The invention relates to a sampling method for a micro-separation system, in particular to a micro-flow liquid chromatography (HPLC) online large-volume sampling method and a special device. Background technique [0002] Since the 1990s, with the development of science and technology and the progress of processing technology, HPLC technology has made new progress. In addition to conventional separation methods, micro-fluidic liquid crystals such as micro-column HPLC, capillary HPLC and microfluidic chip HPLC have also been developed. liquid chromatography separation technique. Compared with conventional HPLC, these micro-separation techniques have lower flow rates (usually in the range of microliters or even nanoliters), smaller solvent consumption and higher separation efficiency. In addition, it can also be used with more types of detectors, such as mass spectrometers. It is suitable for the analysis of complex samples. [0003] Due to the small i...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/16G01N30/60B01J20/281
Inventor 张丽华段继诚张洁张维冰梁作成梁振张玉奎
Owner 中科榆林能源技术运营有限责任公司
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