Preparation method of polyaniline modified electrode for recognizing dead-live cell

Abstract

The invention provides a preparation method of polyaniline-modified electrode for identifying dead-live cells, which solves the problems of the prior art such as complex and time-consuming process, or larger error, and difficulty in identifying dead and live cells and in online operation. The method is achieved via the following steps of: disposing a three-electrode system including home-made graphite powder-epoxy resin solid-state carbon paste as the basic electrode, a saturated calomel electrode as the reference electrode and Pt filament as the inter-electrode in aniline-containing sulfuric acid solution within a potential range of -0.45 to 1.0 V, carrying out cyclic volt-ampere scanning at a speed of 50 mV.s<-1> to electrochemically polymerizing aniline on the graphite powder-epoxy resin solid-state carbon paste to obtain polyaniline-modified electrode; and electrochemically processing the polyaniline-modified electrode to obtain the polyaniline-modified electrode for identifying dead-live cells. The invention has the advantages of easy preparation method, convenient operation and gentle experimental conditions. The modified electrode can be used for identifying, screening and separating dead and live cells, for counting live cells, and for monitoring the growth or usage process of cells in real time since the electrode can reversibly adsorb the live cells.

Description

Technical field: [0001] The invention relates to the field of biomaterials related to cells and the field of bioelectrochemistry, in particular to a method for preparing a polyaniline-modified electrode for identifying dead-living cells, and the electrode prepared by the method can be applied to electrochemical counting of cells , Electrochemical monitoring of cell growth process, electrochemical research of cells, and electrochemical recognition of cells. Background technique: [0002] Changes in the shape and movement state of cells attached to the electrodes will cause changes in the impedance of the adherent interface. Giaever et al. designed a cell that can track the shape changes of mammalian cells in real time, continuously and quantitatively based on the electrical characteristics of the adherent interface. Cell shape analysis sensor. In view of the fact that there are few special reports on the cell counting methods of mammalian embryos, and the cell counting metho...

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Problems solved by technology

[0003] In order to overcome the problems of tedious, time-consuming or large errors in the above methods, it is difficult to distinguish dead cells from living cells, and cannot be monitored online, the present invention provides a method for preparing a polyaniline modified electrode for identifying dead-living cells , the method is realized according to the following steps: under the three-electrode system with self-made graphite powder-epoxy resin solid carbon paste as the basic electrode, saturated calomel electrode as the reference electrode, and platinum wire as the counter electrode, the -0.45~1.0V potential range built in sulfuric acid solution containing aniline, with 50mV.s -1 Carry out cyclic voltammetry scanning, electrochemically polymerize aniline on the graphite powder-epoxy solid carbon paste electrode to obtain a polyaniline modified electrode; then electrochemically treat the obtained polyaniline modified electrode to obtain a polyaniline that recognizes dead-living cells Aniline Modified Electrode

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