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Application of purslane amide alkaloid in preparing antioxidant and neuroprotective agent

A technology of purslane amide and neuron protection, which is applied in the fields of anti-toxic agents, nervous system diseases, neuromuscular system diseases, etc., and can solve the problems that the antioxidant and neuroprotective activities have not been reported.

Inactive Publication Date: 2010-04-14
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Portulaca oleracea amides A, B, and E are amide alkaloids with novel structures isolated by the applicant in 2005 [Xiang L, Xing DM, Wang W, et al. Alkaloids from Portulaca oleracea L..Phytochemistry , 2005, 66(21): 2595-2601], its structure is as follows, but so far, the antioxidant and neuroprotective activities of this type of alkaloids and their application as drugs have not been reported

Method used

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  • Application of purslane amide alkaloid in preparing antioxidant and neuroprotective agent
  • Application of purslane amide alkaloid in preparing antioxidant and neuroprotective agent
  • Application of purslane amide alkaloid in preparing antioxidant and neuroprotective agent

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Embodiment 1: Portulaca oleracea amide alkaloids are used as novel antioxidants

[0041] 1. Experimental Materials and Instruments

[0042] The monomers of purslane amide A, purslane amide B and purslane amide E are extracted and separated from purslane medicinal materials; picryl-hydrazyl, DPPH), vitamin E (α-Tocopherol) and butylated hydroxyanisole (butylated hydroxy anisol, BHA) were purchased from Sigma Company; 1,1,3,3-tetraethoxypropane, thiobaby 2-thiobarbituric acid (TBA) and sodium dodecyl sulfate (SDS) were purchased from Shanghai Sinopharm Chemical Reagent Co., Ltd.; bovine serum albumin and Coomassie brilliant blue G-250 were purchased from Beijing Huamei Transduction Technology Co., Ltd., and other reagents were of analytical grade. The TU-1800 ultraviolet spectrophotometer is produced by Beijing Puxi General Instrument Co., Ltd.; the German-made ULTRA-TURRAX high-shear dispersing emulsifier (homogenizer). Male Wistar rats were provided by the Experiment...

Embodiment 2

[0053] Embodiment 2: Portulaca oleracea amide alkaloids are used as novel neuron protective agents

[0054] 1. Experimental Materials and Instruments

[0055] MEM medium and F12 medium (Gibco), calf serum and fetal bovine serum were purchased from Hangzhou Sijiqing; polylysine and MTT were Sigma products; D-glucose was imported from Sigma; LDH kits were purchased from Beijing Zhongsheng Biological High-Tech Co., Ltd., and the rest of the reagents were domestic analytical grade. Cell culture plates and dishes are Costar products. SANYO (MCO-15AC) carbon dioxide incubator, Olympus inverted microscope and fluorescence microscope; SORVALL refrigerated high-speed centrifuge, T52 ultraviolet-visible spectrophotometer (OHAUS), Bio-RAD microplate reader.

[0056] 2. Experimental steps

[0057] (1) Neuronal cell culture

[0058] Newborn mice (0-3 days) were suffocated to death, sterilized with 75% alcohol for 5 minutes, placed in an ice bath in an ultra-clean bench to separate the ...

Embodiment 3

[0068] Example 3: Portulaca oleracea amide alkaloids are used as ion channel antagonists in neuroprotection

[0069] 1. Experimental Materials and Instruments

[0070] MEM medium and F12 medium (Gibco), calf serum and fetal bovine serum were purchased from Hangzhou Sijiqing; PI, EB, polylysine, Coomassie brilliant blue G250 were Sigma products; D-glucose was imported from Sigma Product; ATPase kit (Nanjing Jiancheng Institute of Bioengineering), and the rest of the reagents were of domestic analytical grade. Cell culture plates and dishes are Costar products. SANYO (MCO-15AC) carbon dioxide incubator, Olympus inverted microscope and fluorescence microscope; SORVALL refrigerated high-speed centrifuge, ZS semi-automatic biochemical analyzer, T52 ultraviolet-visible spectrophotometer (OHAUS), Bio-RAD microplate reader.

[0071] 2. Experimental steps

[0072] The effect of drugs on the ATPase activity on the cell membrane of neurons damaged by hypoxia and glucose: the neurons w...

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Abstract

The invention discloses an application of an oleracein alkaloid in preparing antioxidants, neuroprotective agents, ion channel blockers of brain neuronal cells and inhibitor of apoptosis of brain neuronal cells, wherein the oleracein alkaloid refers to the monomer of oleracein A, oleracein B or oleracein E. The application relates to the development of oleracein alkaloid, which supplies a powerfulbasis for treating neurodegenerative diseases and lays a foundation for further development of the oleracein alkaloid.

Description

technical field [0001] The invention relates to the pharmaceutical application of purslane amide alkaloids, in particular to the application of purslane amide alkaloids in the preparation of antioxidants and neuron protective agents, belonging to the medical field. Background technique [0002] Neurodegenerative diseases are a class of chronic, progressive neurological diseases. Such diseases mainly include Alzheimer's disease, Parkinson's disease, Huntington's chorea, amyotrophic lateral sclerosis and spinal muscular atrophy. With the acceleration of the aging process of the world's population, such neurodegenerative diseases (such as Alzheimer's disease and Parkinson's disease) are increasingly harmful to the health of the elderly. In the United States and Europe, 10% of 65-year-olds and nearly 40% of 85-year-olds suffer from dementia, and its morbidity and mortality are second only to cardiovascular and cerebrovascular diseases and cancer. Although the research on such ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/706A61K31/4745A61K36/185A61P21/00A61P25/14A61P25/16A61P25/28A61P39/06
Inventor 向兰杨子娟
Owner SHANDONG UNIV
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