Method for preparing optical activity alcohol by using yeast cell to catalytically reduce aromatic ketone

Abstract

A method for preparing optically active alcohol by yeast cell catalytic reduction aromatic ketone belongs to the technology field of preparing optically active alcohol in organism catalysis method. In the invention, a given amount of cyclodextrin is added into bioconversion system of yeast cell catalyzing unsymmetrical reduction aromatic ketone to form clathrate with aromatic ketone in order to efficiently improve the dissolvability of reaction bottom object in water and reduce properly the inhibitory action of bottom object and outcome for enzyme. The cyclodextrin improves the catalytic reduction efficiency of yeast juice, increases the stereo selectivity for bottom object, improves evidently thepercent conversion of bottom object and the antipode excess value of outcome. When bottom object concentration is 6-12g / L, different mole ratio cyclodextrin is added according to different bottom object, the percent conversion of bottom object aromatic ketone is 50-99and antipode excess value of outcome optically active alcohol is 75-99D:\˜ñθš€“ž\Àó†¸\�˜ÊÙ\2433biba_�˜13_sunquansheng(100“ž)\2433biba_�˜13_sunquansheng(100“ž).txto the product incorporating the invention has high practical application worthiness.

Description

technical field

[0001] The invention discloses a method for preparing optically active alcohols by catalytically reducing aromatic ketones in yeast cells, which belongs to the technical field of preparing optically active alcohols by biocatalysis. Background technique

[0002] Optically active alcohols are important intermediates for the synthesis of chiral compounds such as chiral drugs, pesticides, and food additives. The asymmetric reduction of prochiral ketones by chemical methods is an important way to prepare optically active alcohols. However, with the gradual depletion of ore resources and the increasing environmental pollution, the current biotransformation technology with the characteristics of high efficiency, specificity and green energy saving is in the market. Applications in asymmetric synthesis are gaining more and more attention. Microbial cells contain many stereoselective enzymes. Using the whole cell to catalyze the asymmetric reduction of prochiral keto...