Fatigue resistant sport drink and method of preparing the same
A sports drink, anti-fatigue technology, applied in food preparation, application, food science and other directions, can solve problems such as harm to the body
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experiment example 1
[0020] Experimental Example 1: For H 2 o 2 Protective effect of induced injury in PC12 cells
[0021] 1.1 Materials: PC12 cells were purchased from the American Type Culture Collection (ATCC);
[0022] Newborn bovine serum, DMEM medium dry powder (U.S. Gibco company);
[0023] h 2 o 2 , MTT (American Sigma Company);
[0024] 12-well culture plate (Danish NUNC company);
[0025] NU2500E CO2 incubator (Forma Scientif, UK);
[0026] The tested Chinese medicine samples were provided by the Department of Biology and the Center for Chinese Medicine Research and Development of the Hong Kong University of Science and Technology.
[0027] 1.2 Method
[0028] (1), the cultivation of PC12 cells: the medium is DMEM medium containing 10% fetal bovine serum, 100U / ml penicillin and 100μg / ml streptomycin at 37°C, 50mL / L CO 2 Routine cultivation. After the cells grow to 80% of the bottom area of the culture flask, add the sample of Chinese medicine to be tested, and add H after 2 h...
experiment example 2
[0034] Experimental example 2: Mouse weight-bearing swimming test
[0035] 2.1 Test material
[0036] 2.1.1 Test sample: sports drink puree of the present invention (containing 5 parts by weight of Astragalus, 1 part by weight of Angelica sinensis, 3 parts by weight of Rhodiola rosea, and 0.3 parts by weight of taurine): 12.4g medicine / 50ml, produced by Hong Kong Provided by the Department of Biology and the Research and Development Center of Traditional Chinese Medicine, University of Science and Technology.
[0037] 2.1.2 Positive control drug: Qingchunbao Anti-aging Tablets (main raw materials are red ginseng, rehmannia glutinosa, asparagus, Radix Ophiopogon japonicus, Digupi, Poria cocos): 0.3g / tablet, Zhengda Qingchunbao Pharmaceutical Co., Ltd. (batch number: 0610001)
[0038] 2.1.3 Test dose: The sports drink prescription is 12.4g crude drug / 1000ml beverage, and adults drink 500ml each time, that is, 6.2g crude drug / person / time, based on an adult body weight of 60kg, ...
experiment example 3
[0046] Experimental Example 3: Determination of serum urea nitrogen, lactate dehydrogenase and liver glycogen in exhausted model mice
[0047] 3.1 Test material:
[0048] 3.1.1 Experimental instrument: 7200 Unocal UV spectrophotometer.
[0049] 3.1.2 Kits: Burea nitrogen (BUN) and lactate dehydrogenase (LDH) kits were provided by Zhongsheng Beikong Biotechnology Co., Ltd.
[0050] The same as Experiment 1 2.1
[0051] 3.2 Methods and results:
[0052] Take 72 mice, half male and half female, weighing 18-22g, and randomly divide them into 6 groups, namely blank control group (only fed with equal volume of distilled water, no exhaustion model), model group, and Qingchunbao anti-aging tablet positive control group, sports drink low, medium and high dosage groups, 12 rats in each group. The doses of intragastric administration in each group were the same as 2.1. The blank group and the model group were given the same volume of distilled water for 7 consecutive days. One hour a...
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