Diabetin-1, 6-diphosphonie acid as well as preparation method and application

A diphosphoric acid and fructose technology, applied in the field of biochemistry, can solve the problems of high cost and harmfulness, and achieve the effects of low production cost, wide source of raw materials and high conversion rate

Inactive Publication Date: 2004-08-11
邢台平安糖业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Equally, the raw material sugar that this method uses is pure glucose, and the cost is higher; And used

Method used

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  • Diabetin-1, 6-diphosphonie acid as well as preparation method and application
  • Diabetin-1, 6-diphosphonie acid as well as preparation method and application

Examples

Experimental program
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preparation example Construction

[0064] 1. First, the preparation of raw material fructose:

[0065] The quality requirements of raw corn are starch content ≥ 74%, impurities ≤ 1.0%, moisture content ≤ 14.0, imperfect kernels ≤ 10.0, and moldy kernels ≤ 1.0. High-quality corn is sieved, air-selected to remove impurities, soaked in 0.3-0.5% sulfurous acid solution for 45-50 hours, and treated by wet milling to separate corn protein, germ and corn bran to obtain corn starch slurry.

[0066]Adjust the corn slurry to Baume degree 17-22, pH value 5.8-6.5, add NOVO company's low calcium, low pH liquefaction enzyme, use the liquefaction injector to liquefy at a temperature of 105-110°C, and maintain 95-100°C, 1.5-2h, reach the liquefaction DE value of 12-15%, cool down to 58-62°C after high-temperature 140°C deactivation, adjust the pH value to 3.8-4.8, add complex glucoamylase produced by NOVO for saccharification, 48-60h, reach DE value above 95%, then add diatomaceous earth and activated carbon to degrease and d...

Embodiment 1

[0094] Saccharomyces cerevisiae was selected for amplified culture, and the bacteria were collected after 12 hours. After the ion exchange resin was immobilized, 20% fructose solution was added to 0.38mol / L Na 2 HPO 4 , 0.015mol / L MgSO 4 , adjust the pH to 6.8-7.1, pass through the immobilized yeast column under the condition of 0.1Bv / h at 40-42°C, measure the content of fructose-1,6-diphosphate in the effluent to be 80.3g, and obtain fructose after purification according to the above process - 1,6-diphosphate 71.3g.

Embodiment 2

[0096] Take Candida (Candida) for amplified culture, collect the thalli after 10 hours and immobilize the ion exchange resin, take 21.2% fructose solution and add 0.36mol / L Na 2 HPO 4 , 0.02mol / LMgSO 4 After adjusting the pH to 6.5-7.0, under the condition of 38-40°C, through the immobilized yeast column, the content of fructose-1,6-diphosphate in the effluent was measured to be 78.3g, and the fructose-1,6-diphosphate was obtained after refining according to the above process. Diphosphoric acid 69.2g.

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Abstract

A process for preparing the fructose-1,6-diphosphate features that the solution containing fructose is contacted with immobilized yeast to transform the fructose to fructose-1,6-diphosphate. The fructose-1,6-diphosphate prepared by said process and its usage are also disclosed.

Description

technical field [0001] This invention relates to the field of biochemistry, and more particularly to fructose-1,6-bisphosphate. The invention also relates to a method for preparing fructose-1,6-diphosphate. The invention also relates to the use of fructose-1,6-bisphosphate. Background technique [0002] Fructose-1,6-diphosphate (FDP) is an intermediate product necessary for the maintenance of the human body in the normal functional metabolism of the human body. It can penetrate the cell membrane of the damaged cell and enter the interior of the cell to directly increase energy, improve the energy metabolism of the cell, and accelerate the recovery of the cell. function, quickly relieve the fatigue state of the human body. [0003] Sodium and calcium salts of fructose-1,6-diphosphate can quickly provide energy for cells, regulate the activity of certain enzymes in sugar metabolism, activate cells, and improve the body's resilience. [0004] Pharmacologically, exogenous FDP...

Claims

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Application Information

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IPC IPC(8): C07H11/04C12P19/02
Inventor 赵建安付海峰
Owner 邢台平安糖业有限公司
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