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Preparation animal protolysate

An animal hydrolyzed protein and animal technology, applied in the field of enzymatic protein hydrolysis, can solve the problems of easy loss of autolytic hydrolase activity, easy inactivation of autolytic hydrolase, long reaction time, etc. The effect of improving the state of physical decline and being convenient to take

Active Publication Date: 2011-03-23
康哲(湖南)制药有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are many sources of enzymes in enzymatic hydrolysis. Various hydrolytic enzymes in biological tissues can be used to decompose cell tissues, and exogenous enzymes suitable for the reaction system can also be added to decompose cell tissues. There are many types of hydrolytic enzymes with different activities, and they will also be affected by other characteristics in the body. The activity of autolytic hydrolytic enzymes in the body is easy to lose
Moreover, using the autolyzed hydrolase present in the body to hydrolyze protein has the disadvantages of long reaction time, low efficiency, low yield, and easy inactivation of the autolyzed hydrolase.

Method used

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  • Preparation animal protolysate

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Mix 30kg of beef and 70kg of eggs (eggshell removed) in a container, add 150kg of water, stir evenly, boil for 30 minutes, cool in a water bath to 30°C, add 0.6kg of protease, which is available in the market, and adjust with ammonia water at a temperature of 30°C Hydrolyze at pH 5.0 for 3 hours to obtain a hydrolyzate; filter the hydrolyzate, discard the filter residue, boil the filtrate, refrigerate at 0-5°C for 5 hours, and filter to collect the supernatant. Filtrate to obtain animal hydrolyzed protein concentrate. Spray drying: spray drying under the conditions of air inlet temperature 165-170°C and outlet air temperature 100-105°C to obtain white protein powder. Directly divided into 5 / bag, 10 / bag, 15 / bag, and packed into finished powder.

Embodiment 2

[0021] Mix 40kg of beef and 60kg of eggs (eggshell removed) in a container, add 300kg of water, stir evenly, boil for 30 minutes, cool in a water bath to 45°C, add 0.3kg of protease, and adjust the pH to 8.0 with ammonia water at a temperature of 45°C Under water hydrolysis for 3 hours to obtain a hydrolyzate; filter the hydrolyzate, discard the filter residue, boil the filtrate, refrigerate at 0-5°C for 5 hours, and collect the supernatant by filtration. Spray-drying: Spray-dry under the conditions of air inlet temperature 165-170°C and outlet air temperature 100-105°C to obtain animal hydrolyzed protein powder.

[0022] Animal hydrolyzed protein powder. Divide into capsules with 0# capsules. 40 capsules / bottle, 100 capsules / bottle can be packaged as a finished product.

Embodiment 3

[0024] Mix 10kg of crocodile meat and 90kg of eggs (eggshell removed) in a container, add 300kg of water, stir evenly, boil for 30 minutes, cool in a water bath to 40°C, add 30kg of pancreatin (porcine pancreatin or animal pancreatin, a commercially available product) ), at a temperature of 38-40°C, hydrolyzed for 3 hours under the condition of pH 6.5 adjusted with ammonia water, to obtain a hydrolyzate; filter the hydrolyzate, discard the filter residue, boil the filtrate, refrigerate at 0-5°C for 12 hours, and filter the supernatant liquid. Filtration, spray drying: spray drying under the conditions of air inlet temperature 165-170°C and outlet air temperature 100-105°C to obtain white animal protein hydrolyzate powder.

[0025] Animal hydrolyzed protein powder 20%, sucrose powder 80%, add and mix appropriate amount of pharmaceutical adjuvants to make granules, dry at 50-80°C, granulate, and pack in 10g / bag, 15g / bag. Packed into finished granules.

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Abstract

The invention discloses a preparation method of hydrolyzed animal protein, which is characterized in that: animal protein with weight account of 10 to 90 and egg protein with weight account of 10 to 90 are mixed in a vessel before stirred evenly through adding water 0.5 to 5 times amount, boiled and cooled; then proteinase or pamcreatin is added for hydrolysis, thereby getting hydrolysate; the hydrolysate is filtered to get the filtrate which is boiled before frozen to get supernatant; concentration and filtering is made to get hydrolyzed protein concentrate solution which is atomized and dried to get hydrolyzed animal protein. Total nitrogen content of the hydrolyzed animal protein can reach more than 12%, rate of amino acid reaches more than 8%. The invention can be used as clinical medication or additive of nutrition food with properties of high nutrition, easy absorption and easy popularization. The invention can be clinically used for hypoproteinemia and malnutrition and systemicfailure caused by each disease and also can be used for burn, fracture and poor wound healing for postoperative wound. The hydrolyzed animal protein has the advantages of obvious therapeutic effect, good taste, convenient use, big market requirement and remarkable economic benefit.

Description

technical field [0001] The invention relates to a method for enzymatically hydrolyzing protein, in particular to a method for preparing animal hydrolyzed protein. Background technique [0002] At present, the protein hydrolysis technology at home and abroad mainly adopts three kinds of process methods: acid hydrolysis method, alkali hydrolysis method and enzyme hydrolysis method. Among them, acid hydrolysis and alkali hydrolysis belong to chemical hydrolysis, while enzymatic hydrolysis belongs to biological enzyme hydrolysis. The acid hydrolysis method is usually hydrolyzed with a strong acid, namely sulfuric acid or hydrochloric acid. The molar concentration of the acid is 6 mol / liter, and the hydrolysis is carried out for more than 20 hours at a high temperature of 110°C. Afterwards, asparagine and glutamic acid were completely hydrolyzed into aspartic acid and glutamic acid respectively, and tryptophan was completely destroyed; serine and threonine were partially hydroly...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23J3/04A23J3/34A23L1/304A61K38/01A23L33/16
Inventor 钟恒费善芬
Owner 康哲(湖南)制药有限公司
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