Membrane dialysis reactor for cultivating intestinal flora

Abstract

The invention discloses a membrane dialysis reactor used for cultivating the intestinal flora. The membrane dialysis reactor comprises a container and a hollow fiber membrane inserting element; the container is divided into a cultivating room and a temperature controlling room, the hollow fiber membrane inserting element comprises a tube pass and a shell pass containing a hollow fiber tube, the hollow fiber membrane inserting element is arranged in the cultivating room, the dialysis liquor inlet opening and the dialysis liquor outlet opening are all arranged outside the cultivating room, the membrane dialysis reactor can realize coexisting of the 3-D cultivation, the suspending cultivation and the anchoring cultivation of the flora simultaneously, the membrane flora and the cavity flora can be simulated, the absorbing and discharging of the metabolic products of the water and the small molecule is more adjacent to the growing state of the flora in the intestinal canal, the density of the cultivation can be increased, the cultivating mode is superior to the traditional cultivating mode, the continuous cultivation and the fed-batch cultivation can be performed by utilizing the reactor, the total number of the anaerobic bacteria displayed by the bacterial cell counting result is about higher 10 times than the common chemostat model, the densities of the bacterium such as the bifidobacterium, lactobacillus and the bacteroid etc are all higher than the chemostat model, the stability and the controllability are strong, the application scope is wide, and the membrane dialysis reactor is suitable for the short / long-term study.

Description

technical field [0001] The invention relates to a bioreactor, in particular to a membrane dialysis reactor for cultivating intestinal flora. Background technique [0002] The human body can be regarded as a "superorganism", which has its own eukaryotic cells and bacteria, archaea and fungi that live in the skin, lungs, digestive tract, vagina and other organs that communicate with the outside world. This is a long-term evolution process. result of natural selection. The number of microorganisms growing in the human body is 10 times that of its own cells, and the number of genes is 100 times that of human genes. There are 500-1000 species of bacteria in the intestinal tract alone, accounting for 30-50% of the total weight of feces. The human body provides a habitat for these microorganisms, and the flora can digest polysaccharides and other substances that the host cannot digest, which can be absorbed and utilized by the host; the flora can also stimulate the development of...

AI Technical Summary

Problems solved by technology

The human intestinal flora is the ecosystem with the highest population density known. Due to the particularity of the living environment of the intestinal flora, it is difficult to simulate the growth of the intestinal flora

Existing methods for simulating intestinal flora mainly include batch culture and continuous culture, also called chemostat model, fed-batch culture, etc. The batch culture method is simple and easy to implement, and the cost is low, but it cannot control the pH value and cannot carry out long-term culture; the continuous culture method and fed-batch culture method can partially simulate the growth environment of the flora, but cannot simulate the host's response to short chain fatty acids (short chain fatty acids). acid, SCFA) and other bacterial metabolites, and the flora is not easy to form a biofilm or the area where the biofilm is formed is too small, resulting in a lower bacterial concentration than that in the intestinal tract

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