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Gene TAP1 relevant to immune trait in pig method for preparing same and applications

A gene and trait technology, applied in the direction of DNA preparation, botany equipment and methods, biochemical equipment and methods, etc., can solve the problems that have not been fully studied on the function of TAP1 gene, so as to improve resistance and reduce the occurrence of diseases Effect

Inactive Publication Date: 2008-10-15
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But so far, there is still no report on the comprehensive study of the function of the TAP1 gene

Method used

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  • Gene TAP1 relevant to immune trait in pig method for preparing same and applications
  • Gene TAP1 relevant to immune trait in pig method for preparing same and applications
  • Gene TAP1 relevant to immune trait in pig method for preparing same and applications

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Embodiment 1

[0033] The present invention will be further described in detail below in conjunction with the accompanying drawings and specific embodiments. Example 1 Preparation of gene TAP1 related to pig immune traits

[0034] The specific experimental process is described as follows:

[0035] 1. Primer Design

[0036] According to the publicly reported mRNA sequence (GenBank Accession: DQ227990) BLASTN analysis, a corresponding porcine genome clone sequence was found, Accession: BX323833. Homologous comparison of the two sequences presumed that the porcine TAP1 gene contains 11 exons. According to the above comparison results, the approximate range of the exons was confirmed and related primers were designed. The reference template for forward and reverse primer design is the porcine BAC clone sequence BX323833, the clone number is XX-554F3, and the primer sequences are:

[0037] Forward: 5'-TCGGAAATGTGGATAAGAGCA-3',

[0038] Reverse: 5'AAACAGACGGATAATGAAAGAGG-3'.

[0039] 2. PCR-RFL...

Embodiment 2

[0048] Example 2 Association analysis of marker traits

[0049] The experimental procedure is attached figure 1 As shown, at first carry out family design, the experimental pig group of the present invention comes from the landrace pig of Guangdong Huanong Wen's Animal Husbandry Co., Ltd. Shuitai original breed pig farm, parent generation is 17 boars and 36 sows, offspring generation altogether 306 piglets , all parents and offspring were tested for genotype; blood samples were collected before and after immunization of offspring, immune indicators were measured, genomic DNA was extracted from parents and offspring, and genotype detection of marker genes was carried out; association analysis.

[0050] Anticoagulant and non-anticoagulant blood were collected from offspring at 1, 17, and 32 days of age for blood routine and antibody level detection.

[0051] The traits analyzed were mainly immune traits. According to the population structure of the collected samples, the appl...

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Abstract

The invention discloses a gene TAP1 related to an immune trait in pigs, the sequence of which is shown in SEQID NO: 1 and in which a base mutant site capable of influencing the immune trait in pigs is detected. The invention also discloses a method for getting the same. The gene and the result of the detected base mutant site provided by the invention can be use to provide a novel molecular marker for the marker assisted breeding of pigs. The invention is expected to improve the resistibility of livestock and poultry in essence and reduce the occurrences of diseases, which is critical to disease control, and improve the benefits and safety of breeding pigs both on the surface and at the root.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering, and specifically relates to a porcine immune trait-related gene TAP1 and a preparation method thereof, a mutation site detection method thereof, and an application in establishing molecular markers suitable for porcine immune traits. Background technique [0002] In the past 10 years, modern breeding technology has significantly improved pig production performance (growth, carcass and meat quality traits), but insufficient attention has been paid to the genetic improvement of pig health and disease resistance. [0003] With the rapid development of molecular genetics, the continuous improvement of genetic maps of livestock and poultry, and the continuous supplement of genetic markers, some genes or genetic markers related to disease resistance have been discovered one after another. The use of genetic methods to improve the resistance of livestock and poultry from the genetic nature a...

Claims

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Application Information

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IPC IPC(8): C12N15/12C12N15/10C12Q1/68
Inventor 吴珍芳孙奴奴陈慧勇
Owner SOUTH CHINA AGRI UNIV
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