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Adsorbing agent for blood perfusion adsorbing bilirubin in vitro and preparation

An extracorporeal blood and adsorbent technology, which is applied to chemical instruments and methods, suction devices, and other chemical processes, can solve the problems of high price and restrictions on the application of such products, and achieve strong adsorption capacity, excellent adsorption rate, and blood phase good capacitive effect

Inactive Publication Date: 2008-11-05
TIANJIN DEOUTE BIOLOGY MEDICINE SICENCE & TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] At present, there is no special safe and effective bilirubin adsorbent product in our country, and Japanese products are expensive, which greatly limits the application of such products in our country

Method used

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  • Adsorbing agent for blood perfusion adsorbing bilirubin in vitro and preparation
  • Adsorbing agent for blood perfusion adsorbing bilirubin in vitro and preparation
  • Adsorbing agent for blood perfusion adsorbing bilirubin in vitro and preparation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment 1, polyvinyl alcohol microspheres are the preparation of the adsorbent of carrier

[0031] a. Preparation of polyvinyl alcohol microspheres

[0032] Add 24g of vinyl acetate, 6g of triallyl isocyanurate, 15ml of ethyl acetate, 15ml of n-heptane and 1.6g of polyvinyl acetate (polymerization degree 500) into a 500ml three-necked flask, and the system is heated to 40°C. Mechanical stirring, add 0.3g initiator azobisisobutyronitrile after the polyvinyl acetate dissolves completely, add 300ml aqueous solution (by weight, containing 1.5% polyvinyl alcohol and 3% chlorine Sodium chloride), adjust the stirring speed to make the droplets uniformly dispersed and suitable in size, slowly raise the temperature to 65°C, keep the temperature and stirring speed for polymerization for 3 hours, then raise the temperature to 75°C for another 3 hours to obtain a white granular copolymer. Filter, wash with hot water, extract with acetone in a Soxhlet extractor for 12 hours, and...

Embodiment 4

[0037] Example 4, the fixation of 1,6-hexanediamine functional group: take 1ml of the activated microspheres obtained in the step of Example 1b, add 0.75ml 2mol / L NaOH, 5ml 1,6-hexanediamine, at 75°C Shake overnight, and then fully wash with distilled water to obtain a bilirubin adsorbent for use.

Embodiment 5

[0038] Embodiment 5, adsorbent static adsorption experiment in vitro: measure 0.5ml respectively by embodiment 2, embodiment 3, the adsorbent prepared in embodiment 4, place in the polypropylene plastic tube of 10.0ml, add appropriate amount of normal saline Equilibrate for 3 hours, absorb free physiological saline, add 5.0ml of bilirubin simulated serum solution with a concentration of 342μmol / L (a certain amount of bilirubin is dissolved in PBS buffer, which contains 20g / L albumin), 37°C Shake at constant temperature for 3 hours, let it stand for a while, absorb the supernatant to measure the concentration of bilirubin, and set a blank control, the adsorption rate is calculated by the following formula:

[0039] AP = ([C] B -[C] A ) / [C] B ×100%

[0040] Where AP is the adsorption rate (%), [C] B and [C] A Concentrations before and after adsorption (mg / dl), respectively.

[0041] By embodiment 2, embodiment 3, the adsorption rate of the adsorbent that embodiment 4 makes...

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Abstract

The invention relates to an adsorbent used for adsorbing cholerythrin in extra corporeal blood perfusion and a preparation method thereof. The method takes polyvinyl alcohol microspheres with good biological compatibility as a carrier, adopts epoxy chloropropane for activation and fixes an amination agent as a functional group to form the cholerythrin adsorbent. The surface of the adsorbent is provided with a plurality of positive charges, which is beneficial to action with with the cholerythrin provided with negative charges on the surface to generate absorption effect. The adsorbent prepared by the invention has good biological compatibility and good adsorbing capacity, and is characterized by low manufacturing cost, simple synthesis process, large adsorbing volume, and high adsorbing speed, etc., and can be used in extra corporeal blood perfusion for curing hyperbilirubinemia.

Description

【Technical field】 [0001] The invention belongs to the technical field of biomedicine. It relates to a novel medical adsorbent, especially an adsorbent used for extracorporeal hemoperfusion to remove excessively high bilirubin in blood. 【Background technique】 [0002] Bilirubin (Bilirubin, BR) is a kind of bile pigment, a metabolite of aging red blood cells. In the body, bilirubin is generally combined with glucuronic acid to form a water-soluble complex, which is transported by the blood to the liver for metabolism, and passes through the biliary tract. excretion. If there is too much bilirubin in the body, or liver damage, bilirubin blockage and other reasons can cause a large amount of bilirubin to accumulate in the body, resulting in hyperbilirubinemia, the bilirubin level in severe patients can reach 700 μmol / L . Free bilirubin is an endogenous toxin that can cause abnormal functions of organs such as the liver and biliary tract. Due to its fat solubility, it can easi...

Claims

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Application Information

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IPC IPC(8): B01J20/26B01J20/30A61M1/00
Inventor 孔德领顾汉卿王为超张胜男俞耀庭欧来良
Owner TIANJIN DEOUTE BIOLOGY MEDICINE SICENCE & TECH
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