Method for biological catalysis preparation of (R)-2-hydroxyl-4-phenyl ethyl butyrate

A technology of ethyl phenylbutyrate and biocatalysis, applied in biochemical equipment and methods, methods based on microorganisms, microorganisms, etc., can solve the problem of lack of high enantioselectivity and high yield microbial enzyme sources, low conversion rate, etc. problem, to achieve high enantiomeric value, high optical purity, high reaction conversion effect
CN101314784AInactive Publication Date: 2008-12-03重庆博腾精细化工有限公司
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
重庆博腾精细化工有限公司
Publication Date
2008-12-03
Estimated Expiration
Not applicable · inactive patent
Patent Text Reader

Abstract

The invention relates to a method for preparing (R)-2-hydroxyl-4- phenylbutyric ethyl butyrate by adopting biological catalysis, which belongs to the technology field of the biocatalytic anisomerous reducing preparation medical chiral intermediate. The method comprises the following steps: utilizing produced bacteria Candida boidinii of high antimer selective carbonyl reductase screened etc.; fermenting and producing enzyme under the optimizing condition; taking 2-hydroxyl-4-phenylbutyric ethyl butyrate as a substrate in a single water phase system, a water / organic two-phase system or a water / resin two-phase system; adding grape sugar to wet thalli; and preparing (R)-2- hydroxyl-4- phenylbutyric ethyl butyrate. When the concentration of the substrate of 2-hydroxyl-4-phenylbutyric ethyl butyrate is 1 to 50g / L, (R)-2- hydroxyl-4- phenylbutyric ethyl butyrate is subjected to transformation for 1h to 48h, so as to ensure that the enantiomeric excess of the (R)-2- hydroxyl-4- phenylbutyric ethyl butyrate reaches 84.9 to 98.88 percent, the transformation rate of Moore reaches 72.0 to 84.6 percent, and coenzyme is not needed during the whole reaction process.
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Description

technical field

[0001] The invention relates to a biocatalytic method for preparing ethyl (R)-2-hydroxy-4-phenylbutyrate, and belongs to the technical field of biocatalytic asymmetric reduction preparation of medical chiral intermediates. Background technique

[0002] Chiral (R)-2-hydroxy-4-phenylbutanoic acid ethyl ester (Benzenebutanoic acid, a-hydroxy-, ethylester, (aR)-), the molecular formula is C 12 h 16 o 3 , molecular weight 208.25, CAS number: 90315-82-5. (R)-2-Hydroxy-4-phenylbutyric acid ethyl ester is a key chiral intermediate in the synthesis of many angiotensin-converting enzyme inhibitors (ACEI) pril drugs. Due to the importance of ethyl (R)-2-hydroxy-4-phenylbutyrate in the synthesis of this class of drugs, many people have been attracted to conduct extensive research on its preparation, and a large number of preparation methods have been reported.

[0003] At present, the production of ethyl (R)-2-hydroxy-4-phenylbutyrate can be carried out by chemical o...

Claims

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