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Kalium diagnosis / determination reagent kit and method for determining kalium concentration

A method of determination, technology of potassium concentration, applied in the fields of medical/food/environmental inspection and determination, which can solve the problems of expensive electrodes

Inactive Publication Date: 2008-12-24
SUZHOU ANJ BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the ion selective electrode method is widely used, but the cost of the electrode is expensive.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] The potassium diagnosis / measurement reagent of the present embodiment is a single reagent, comprising:

[0047] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0048] Stabilizer 500mmol / L

[0049] Reduced coenzyme 0.25mmol / L

[0050] Pyruvate kinase 6000U / L

[0051] Alanine dehydrogenase 8000U / L

[0052] Glycine oxidase 10000U / L

[0053] Adenosine diphosphate 8mmol / L

[0054] Phosphoenolpyruvate 3mmol / L

[0055] Ammonia ion 20mmol / L

[0056] After the reagents are all dissolved and prepared, they are divided into bottles and freeze-dried to make dry powder reagents; before use, add purified water and reconstitute for use.

[0057] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance 1.8±0.7, test main wavelength 340nm, test secondary wavelength 405nm, the volume ratio of the tested potassium sample to the reagent is 1 / 25, The reaction direction is negative reaction (decreasing reaction), the...

Embodiment 2

[0060] The potassium diagnosis / measurement reagent of the present embodiment is a double reagent, comprising:

[0061] Reagent 1

[0062] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0063] Stabilizer 50mmol / L

[0064] Reduced coenzyme 0.25mmol / L

[0065] Adenosine diphosphate 8mmol / L

[0066] Phosphoenolpyruvate 3mmol / L

[0067] Ammonia ion 20mmol / L

[0068] Reagent 2

[0069] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0070] Stabilizer 500mmol / L

[0071] Pyruvate kinase 6000U / L

[0072] Alanine dehydrogenase 8000U / L

[0073] Glycine oxidase 10000U / L

[0074] After the reagents are all dissolved and prepared, they are divided into bottles to make liquid double reagents, which can be used directly.

[0075] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance 1.8±0.7, test main wavelength 340nm, test secondary wavelength 405nm, the volume ratio of the potassium sam...

Embodiment 3

[0078] The potassium diagnosis / measurement reagent of the present embodiment is three reagents, comprising:

[0079] Reagent 1

[0080] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0081] Stabilizer 50mmol / L

[0082] Reduced coenzyme 0.25mmol / L

[0083] Adenosine diphosphate 8mmol / L

[0084] Phosphoenolpyruvate 3mmol / L

[0085] Ammonia ion 20mmol / L

[0086] Reagent 2

[0087] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0088] Stabilizer 500mmol / L

[0089] Alanine dehydrogenase 8000U / L

[0090] Glycine oxidase 10000U / L

[0091] Reagent 3

[0092] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0093] Stabilizer 500mmol / L

[0094] Pyruvate kinase 6000U / L

[0095] After all the reagents are dissolved and prepared, they are divided into bottles to make liquid three reagents, which can be used directly.

[0096] When measuring the potassium concentration, set on the automatic biochemical analyzer: t...

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Abstract

The invention relates to a kalium diagnosis / determination kit which uses the techniques of an enzyme circulation amplification method, an enzyme colorimetry method and an enzyme-linked method; simultaneously, the invention also relates to a method principle used for determining kalium consistency, reagent composition and component, belonging to the field of medical / food / environment detection determination technique. The main compositions of the kit of the invention comprise buffer solution, reduction-typed coenzyme, adenosine diphosphate, phosphoenolpyruvate, ammonium ion, pyruvate kinase, alanine dehydrogenase, glycine oxidase, and stabilizer; samples and reagents are mixed according to a certain volume proportion so as to generate a series of enzymatic reactions; subsequently, reaction matters are arranged under an ultraviolet / visible light analyser so as to detect the degree / speed of the absorbency reduction of the main wavelength at the 340nm position, thus calculating the consistency of the kalium.

Description

technical field [0001] The invention relates to a potassium diagnosis / measurement kit, and meanwhile, the invention also relates to a method for measuring potassium concentration, which belongs to the technical field of medicine / food / environmental inspection and determination. Background technique [0002] Clinical significance of three decisive levels of serum potassium: below the lower limit of the reference value, showing hypokalemia due to renal tubular disease, hyperaldosteronism, hyperinsulinism, metabolic alkalosis, diuretic therapy, insulin therapy for diabetes During ketosis, potassium ions are transferred into cells, etc. If it is higher than the upper limit of the reference value, it may be caused by glomerular disease, renal insufficiency, diabetic ketosis, adrenal cortical insufficiency, etc. When the serum potassium is as high as 7.5mmol / L or above, the patient will experience arrhythmia, and appropriate treatment should be considered. When serum potassium ex...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/84G01N21/31C12Q1/26C12Q1/00
Inventor 王尔中
Owner SUZHOU ANJ BIOTECHNOLOGY CO LTD
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