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Preparation technique of protein microsphere suppository

A production process, protein technology, applied in drug delivery, non-active ingredients of polymer compounds, non-active ingredients of oil/fat/wax, etc., can solve problems such as inflammatory response and toxicity

Inactive Publication Date: 2008-12-31
WUHAN INSTITUTE OF TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, common cross-linking agents (such as glutaraldehyde, formaldehyde, etc.) have certain toxicity, and the residual cross-linking agent in the microsphere embolism prepared by the method of curing with these cross-linking agents is easy to cause the inflammatory response of the human body

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] Example 1: Preparation of gelatin microspheres

[0012] Raw materials and sources

[0013] Gelatin [China National Pharmaceutical Group Shanghai Chemical Reagent Company]

[0014] Distilled water 【Homemade, double distillation】

[0015] Soybean oil 【China Food Products Co., Ltd.】

[0016] Acetone 【Tianjin Fuyu Fine Chemical Co., Ltd.】

[0017] 20ml of gelatin aqueous solution (20%, mass ratio) was added to 100ml of soybean oil for emulsification at a temperature of 50°C, then cooled, washed with acetone to remove the oil, centrifuged, and dried to obtain protein microspheres. Add the dried protein microspheres to soybean oil preheated to 100°C, keep it at this temperature for 24 hours, wash off the oil with acetone, dry, sieving and grading, and then bottling and capping.

Embodiment 2

[0018] Example 2: Preparation of Gelatin Microspheres

[0019] Raw materials and sources

[0020] Gelatin [China National Pharmaceutical Group Shanghai Chemical Reagent Company]

[0021] Distilled water 【Homemade, double distillation】

[0022] Soybean oil 【China Food Products Co., Ltd.】

[0023] Acetone 【Tianjin Fuyu Fine Chemical Co., Ltd.】

[0024] 40ml of gelatin aqueous solution (30%, mass ratio) was added to 100ml of soybean oil for emulsification at a temperature of 60°C, then cooled, washed with acetone to remove the oil, centrifuged, and dried to obtain protein microspheres. Place the dried protein microspheres in an oven preheated to 150°C, keep this temperature for 24 hours, cool, sieving and grading, and then bottling and capping.

Embodiment 3

[0025] Example 3: Preparation of albumin microspheres

[0026] Raw materials and sources

[0027] Albumin 【Wuhan Institute of Biological Products】

[0028] Distilled water 【Homemade, double distillation】

[0029] Peanut oil 【China Food Products Co., Ltd.】

[0030] Acetone 【Tianjin Fuyu Fine Chemical Co., Ltd.】

[0031] 20ml of bovine serum albumin aqueous solution (33%, mass ratio) was added to 100ml of peanut oil for emulsification at a temperature of 50°C, then cooled, washed with acetone to remove the oil, centrifuged, and dried to obtain protein microspheres. Place the dried protein microspheres in a watch glass, then place the watch glass in a sand bath, heat it to 150°C, keep it at this temperature for 24 hours, cool, sieving and grading, and then bottling and capping.

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Abstract

The present invention relates to a manufacture technology of an embolic agent, in particular to a manufacture technology of a protein microsphere embolic agent. The manufacture technology is realized by the following steps: protein aqueous solution is added into vegetable oil for emulsification under the temperature of 40 DEG C to 70 DEG C; protein microspheres are obtained after deoiling and drying, wherein, the volume ratio of the protein aqueous solution and the vegetable oil is 1 to 2:5; then, the obtained protein microspheres are subject to thermal crosslinking to be solidified; the protein microspheres obtained after solidification are sieved and graded or are deoiled, dried, sieved and graded to obtain a microsphere embolic agent in different particle diameters. An emulsification method is used, so the manufactured protein microspheres have regular shapes; the protein microspheres which are heated and solidified can take effect of the protein microspheres which are solidified by a cross linker and do not contain residual cross linker, and the protein microsphere embolic agent is safer to human body and is fit for the interventional therapy better.

Description

Technical field [0001] The invention relates to a manufacturing process of an embolic agent, in particular to a manufacturing process of a protein microsphere embolic agent. Background technique [0002] At present, interventional therapy has been widely used in the treatment of a variety of tumor diseases. Interventional therapy refers to inserting catheters and instruments made of special materials into the tumor site in the body under the guidance of X-rays, and then injecting anti-tumor drugs through the blood supply artery or blocking the blood supply of tumor tissue, so that the tumor necrosis and Atrophy, achieve the purpose of treatment. The use of particulate embolic agents that block the blood supply of tumor tissue is an important means of interventional therapy. Protein particles are one of the most common vascular embolization materials in clinical practice. During their preparation, cross-linking agents are usually used to solidify the protein. However, common cross...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/00A61K47/42A61K47/44A61P35/00
Inventor 吴姝雯陈文斌段蓉柏正武
Owner WUHAN INSTITUTE OF TECHNOLOGY
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