Thunder god vine medicinal materials triptolide content determination method

Abstract

The invention discloses a test method for the content of triptolide in the crude drugs of common threewingnut root; the test method of the triptolide ever reported has poor precision, low recovery rate and big error and the product quality can not be controlled completely and effectively; the invention adopts a high performance liquid chromatography which comprises the following operation steps: the reference solution is prepared; the triptolide reference is taken and methanol is added so as to prepare solution; the test sample solution is prepared; the common threewingnut root powder is taken and added with methenyl choloride, heating, reflow and cooling are then carried out in sequence; the methenyl choloride is added to make up the lost weight, the obtained solution is shaken to be even and filtered; subsequent filtrate is taken and the solvent is dried; the residue is added with the methenyl choloride for fractional dissolution; the obtained mixture is arranged on a processed aluminium oxide column, and is eluted by using ethyl acetate-absolute ethyl alcohol; the elution is collected and evaporated to dryness; the residue is added with the methanol for dissolution, shaken to be even and filtered, and the subsequent filtrate is taken; the reference solution and the test sample solution are taken respectively to be injected into a liquid chromatograph. The invention can control the quality of the crude drugs of the common threewingnut root effectively and ensures the good material of the finished product by the common threewingnut root.

Description

technical field [0001] The invention relates to content determination of substances, in particular to a method for content determination of triptolide in triptolide medicinal materials. Background technique [0002] Tripterygium Wilfordii (Tripterygium Wilfordii Hook.f.), aliased as Broken Heart Grass, Huangteng, and Cabbage Insect Medicine, is a plant of the genus Tripterygium Wilfordii in the family Wetmaniaceae, a deciduous vine-like shrub, which has anti-inflammatory, antibacterial, immune regulation, anti-tumor and anti-fertility properties. And other pharmacological effects, is the first choice of immunosuppressants at home and abroad. A large number of pharmacological and clinical studies have shown that triptolide is the main active component of triptolide, and it is also the main toxic component. At present, the medicinal materials of triptolide are mainly collected from the wild, and the quality is uneven. The main active ingredients such as triptolide are very un...

AI Technical Summary

Problems solved by technology

In the current domestic and foreign relevant literature, the determination method of triptolide in tripterygium wilfordii medicinal material has been reported, but it has been verified by experiments that its precision is poor, the recovery rate is low, and the error is large, so it cannot fully and effectively control the product quality.

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