Simultaneous sequential chemical extraction of jujube flavones and jujube polysaccharide by fermentation method

Abstract

A method for simultaneously extracting jujube polysaccharide and jujube flavone in sequence from jujube is that most part of monosaccharide and disaccharide in jujube is removed by the yeast fermentation method and the jujube polysaccharide and jujube flavone are extracted respectively from fermentation liquid and the fermented jujube residue. Jujube powders and water are extracted according to acertain proportion, are added with activated yeast to carry out the aerobic fermentation to complete the fermentation when the residual sugar content of reducing sugar is 2 to 5 percent (weight); filtrate and filtrate residue are obtained by filtering; the filtrate is concentrated with ethanol precipitation, and is filtered, deproteinized and dried to obtain the jujube polysaccharide. The filter residue is refluxed and extracted by ethanol solution, the extract liquid is reduced in pressure and concentrated, extracted by petroleum ether, removed the petroleum ether and absorbed by a macroporous resin column; eluent is reduced in pressure, concentrated and carried out the vacuum freeze drying to obtain the jujube flavone. The method has the advantages of reduction of the interference on extracting the jujube polysaccharide and the jujube flavone, no pollution, energy saving, synchronous extraction of the jujube polysaccharide and the jujube flavone, low cost of products by one process,and can be volume-produced by industrialization.

Description

technical field [0001] The invention relates to a method for simultaneously extracting jujube polysaccharides and jujube flavonoids by yeast fermentation, and belongs to the technical field of biological products and traditional Chinese medicine preparation. Background technique [0002] Jujube is the ripe fruit of the rhamnaceae plant jujube. Jujube trees are distributed in the vast areas of northern my country, and generally grow in the transition zone between the middle temperate zone and the frigid zone. Jujube trees are also one of the main tree species for returning farmland to forests and developing economic forests and fruits in the middle and lower reaches of the Yellow River in recent years, and the development speed is very fast. Jujube fruit is rich in nutrition. In addition to containing a large amount of sugar, it also contains flavonoids, saponins and 19 kinds of amino acids necessary for human body, a large amount of inorganic salts, multivitamins, dietary fi...

AI Technical Summary

Problems solved by technology

Under normal temperature or acidic conditions, the solubility of flavonoids in water is very low, but under strong alkali conditions, the structure of flavonoids will be destroyed, so the yield of flavonoids in water extraction is low, and it is easy to dissolve proteins, sugars, etc. in water The components are extracted, which makes the subsequent separation work very difficult.

However, organic solvents such as methanol and ethanol can overcome the shortcomings of the water extraction method and are the most commonly used extraction methods. However, due to the relatively high sugar content in jujube, there is a great interference to the extraction and purification of jujube flavonoids, especially It is a monosaccharide and a disaccharide, which brings difficulties to the purification of jujube flavonoids

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